Taq DNA Polymerase

  • Catalog number
    9001-500
  • Price
    Please ask
  • Size
    500 u
  • Synonyms
    DNA polymerease
  • Alternative_names
    DNA polymerase I, thermostable, DNA polymerase I, thermostable (EC 2.7.7.7) (Taq polymerase 1) polA pol1
  • Description
    A thermostable enzyme that catalyzes the polymerization of nucleotides
  • Recombinant
    Yes
  • Source
    E. coli
  • Assay
    SDS-PAGE
  • Unit Definition
    One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nanomoles of dNTPs into acid insoluble material in 30 minutes at 74°C under standard DNA polymerase assay conditions.
  • Molecular Weight
    94.0 kDa
  • Storage Temp
    -20°C
  • Shipping
    gel pack
  • Shelf Life
    12 months
  • Concentration
    2.5 U/µl
  • Appearance
    Liquid
  • Physical form description
    20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol, 0.5% Tween 20, and 0.5% Nonidet P40.
  • Background Information
    Taq DNA Polymerase is a thermostable enzyme that replicates DNA and exhibits a half-life of 40 minutes at 95°C. Taq catalyzes the polymerization of nucleotides into duplex DNA in the 5’ –3’ direction in the presence of magnesium. The enzyme has an apparent molecular weight of 94,000 daltons by SDS-PAGE and exhibits 5’– 3’exonuclease activity. Taq is recommended for use in PCR and primer extension reactions at elevated temperature. The 10X Reaction Buffer provided has been optimized for amplification of both short and long PCR products.
  • Handling
    Centrifuge the vial prior to opening.
  • Usage
    For Research Use Only! Not to be used in humans
  • Gene target
    Taq   DNA   Polymerase  
  • Gene symbol
    PRKDC, HLA-DOA
  • Short name
    Taq DNA Polymerase
  • Technique
    dna, Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. Biovision TAQ has a far higher yield that other polymerases. TAQ is perfect for verificative PCR in an agarose gel. The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. For proofreading mixes with pfu are required. It is supplied in 1
  • Alternative name
    Taq Desoxyribonucleic acid polymerizing enzyme
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