Taq DNA Polymerase
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Catalog number9001-500
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PricePlease ask
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Size500 u
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SynonymsDNA polymerease
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Alternative_namesDNA polymerase I, thermostable, DNA polymerase I, thermostable (EC 2.7.7.7) (Taq polymerase 1) polA pol1
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DescriptionA thermostable enzyme that catalyzes the polymerization of nucleotides
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RecombinantYes
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SourceE. coli
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AssaySDS-PAGE
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Unit DefinitionOne unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nanomoles of dNTPs into acid insoluble material in 30 minutes at 74°C under standard DNA polymerase assay conditions.
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Molecular Weight94.0 kDa
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Storage Temp-20°C
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Shippinggel pack
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Shelf Life12 months
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Concentration2.5 U/µl
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AppearanceLiquid
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Physical form description20 mM Tris-HCl, pH 8.0, 100 mM KCl, 0.1 mM EDTA, 1 mM DTT, 50% glycerol, 0.5% Tween 20, and 0.5% Nonidet P40.
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Background InformationTaq DNA Polymerase is a thermostable enzyme that replicates DNA and exhibits a half-life of 40 minutes at 95°C. Taq catalyzes the polymerization of nucleotides into duplex DNA in the 5’ –3’ direction in the presence of magnesium. The enzyme has an apparent molecular weight of 94,000 daltons by SDS-PAGE and exhibits 5’– 3’exonuclease activity. Taq is recommended for use in PCR and primer extension reactions at elevated temperature. The 10X Reaction Buffer provided has been optimized for amplification of both short and long PCR products.
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HandlingCentrifuge the vial prior to opening.
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UsageFor Research Use Only! Not to be used in humans
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Gene target
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Gene symbolPRKDC, HLA-DOA
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Short nameTaq DNA Polymerase
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Techniquedna, Thermus Aquaticus or TAQ polymerase is a very robust enzyme that lacks endomuclease proof-reading. Biovision TAQ has a far higher yield that other polymerases. TAQ is perfect for verificative PCR in an agarose gel. The bands in which positive primers and a negative control are included need to be compared to a DNA 100 bp molecular weight ladder. For proofreading mixes with pfu are required. It is supplied in 1
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Alternative nameTaq Desoxyribonucleic acid polymerizing enzyme
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Gene info
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Identity
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Gene
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Long gene nameprotein kinase, DNA-activated, catalytic subunit
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Synonyms gene
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Synonyms gene name
- protein kinase, DNA-activated, catalytic polypeptide
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Synonyms
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Synonyms name
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Locus
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Discovery year1993-11-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Armadillo like helical domain containing
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namemajor histocompatibility complex, class II, DO alpha
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Synonyms gene
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Synonyms
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GenBank acession
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Locus
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Discovery year2001-06-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- C1-set domain containing
- Histocompatibility complex
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VEGA ID
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Locus Specific Databases