PolyScience

PolyScience is a U.S.-based manufacturer specializing in liquid temperature control solutions for laboratory, industrial, medical, and culinary applications. Founded in 1963 and headquartered in Niles, Illinois, PolyScience has built a global reputation for innovative, reliable, and environmentally conscious products .

Core Product Categories

PolyScience offers a comprehensive range of temperature control equipment:

Refrigerated & Heated Circulators: Ideal for precise temperature control in laboratory applications, these circulators are designed for both heating and cooling tasks.
Chillers & Recirculating Coolers: These systems provide efficient heat removal for various industrial and laboratory processes.
Water & Viscosity Baths: Used for applications such as warming reagents, thawing samples, and biological incubation.
Application-Specific Products: Including coliform baths, cryoprecipitate baths, and histology freeze plates tailored for specialized laboratory needs .

Browse PolyScience Products

10L Digital Water Bath (Ambient +5° to 99°C), 240V, 50Hz

SKU: WBE10A12E|Size: 1 System

15L Refrigerated Circulator, Advanced Digital (-40° to 200°C), 240V, 50Hz

SKU: AD15R-40-A12E|Size: 1 System

15L Refrigerated Circulator, Advanced Programmable (-40° to 200°C), 240V, 50Hz

SKU: AP15R-40-A12E|Size: 1 System

20L Digital Water Bath (Ambient +5° to 99°C), 240V, 50Hz

SKU: WBE20A12E|Size: 1 System

20L Stainless Steel Open Bath, MX (Ambient +10° to 135°C), 240V, 50Hz

SKU: MX20S135-A12E|Size: 1 System

28L Digital Water Bath (Ambient +5° to 99°C), 240V, 50Hz

SKU: WBE28A12E|Size: 1 System

2L Digital Water Bath (Ambient +5° to 99°C), 240V, 50Hz

SKU: WBE02A12E|Size: 1 System

5L Digital Water Bath (Ambient +5° to 99°C), 240V, 50Hz

SKU: WBE05A12E|Size: 1 System

6L Stainless Steel Open Bath, MX (Ambient +10° to 135°C), 240V, 50Hz

SKU: MX06S135-A12E|Size: 1 System

7L Heated Circulator, MX (Ambient +10° to 135°C), 240V, 50Hz

SKU: MX07H135-A12E|Size: 1 System

7L Low-Profile Refrigerated Circulator, Advanced Digital (-20° to 200°C), 240V, 50Hz

SKU: AD7LR-20-A12E|Size: 1 System

7L Low-Profile Refrigerated Circulator, MX (-20° to 135°C), 240V, 50Hz

SKU: MX7LR-20-A12E|Size: 1 System

7L Space-Saving Refrigerated Circulator, Advanced Digital (-20° to 200°C), 240V, 50Hz

SKU: AD07R-20-A12E|Size: 1 System

7L Space-Saving Refrigerated Circulator, Advanced Digital (-40° to 200°C), 240V, 50Hz

SKU: AD07R-40-A12E|Size: 1 System

7L Space-Saving Refrigerated Circulator, MX (-20° to 135°C), 240V, 50Hz

SKU: MX07R-20-A12E|Size: 1 System

7L Space-Saving Refrigerated Circulator, Standard Digital (-20° to 170°C), 240V, 50Hz

SKU: SD07R-20-A12E|Size: 1 System

AIR FLTR, VINYL,16.5IN X 15.5, CA02-CA10

SKU: 750-967|Size: 1 Each

Air Filter Kit, Dynamic (DuraChill CA Chillers AFTER 9/1/2021)

SKU: 511-550|Size: 1 Each

Air Filter for 07R-20, 07R-40, 15RCAL and Histology Bath

SKU: 305-054|Size: 1 Each

Air Filter for 15R-30, 15R-40, 20R-30, and 28R-30 Refrigerated/Heated Circulating Baths

SKU: 305-055|Size: 1 Each

Air Filter for 2 and 3HP DuraChill® Chillers

SKU: 305-033|Size: 1 Each

Air Filter for 45R-20 and 75L Refrigerated/Heated Circulating Baths

SKU: 305-056|Size: 1 Each

Air Filter for 5, 7.5 and 10HP DuraChill® Chillers

SKU: 750-264|Size: 1 Each

Air Filter for 6000 Series (1/4 to 1HP), Model 3370, IP-100, IP-80, Cryoprecipitate Bath

SKU: 750-855|Size: 1 Each

Air Filter for 7LR-20 Refrigerated/Heated Circulating Baths

SKU: 305-057|Size: 1 Each

Air Filter for LM and MM Benchtop Chillers

SKU: 750-798|Size: 1 Each

Air Filter for LS Benchtop Chillers

SKU: 750-758|Size: 1 Each

Air Filter, Dynamic, (DuraChill CA Chillers)

SKU: 511-535|Size: 1 Each

Base with locking casters for 15, 20, 28 liter Circulators

SKU: 505-194|Size: 1 Each

Base with locking casters for LS/LM

SKU: 505-169|Size: 1 Each

Bypass Kit, for higher temperature, Viton® tubing, 1/2” x 6” with spring & 2 hose clamps

SKU: 510-495|Size: 1 Each

Bypass Kit, replacement, Buna N tubing, 1/2” ID x 6” with spring and insulation

SKU: 510-711|Size: 1 Each

Bypass Kit, silicone tubing, 1/2” ID x 6”, MX Controllers only

SKU: 510-713|Size: 1 Each

CHILLER -10C 120/60 G3 PSC

SKU: BA05A1G3-110A1NC|Size: 1 Each

CHILLER -10C 220-240/50 G3 PSC

SKU: BA05A0G3-110A1NE|Size: 1 Each

CHILLER -20C 120/60 G3 PSC

SKU: BA06A1G3-110A1NC|Size: 1 Each

CHILLER -20C 120/60 G3 UV PSC

SKU: BA06A1G3-310A1NC|Size: 1 Each

CHILLER -20C 220-240/50 G3 PSC

SKU: BA06A0G3-110A1NE|Size: 1 Each

CHILLER -20C 220-240/50 G3 UV PSC

SKU: BA06A0G3-310A1NE|Size: 1 Each

Cable, Ethernet, 7’ (2.1 m)

SKU: 225-670|Size: 1 Each

Cable, RS232, 9.8’ (3 m)

SKU: 225-173|Size: 1 Each

Cable, USB-A to Mini-B, 6’ (1.8 m)

SKU: 225-669|Size: 1 Each

Calibration Bath, Refrigerated, 15L, Advanced Programmable, (-30° to 200°C), 120V, 60Hz

SKU: AP15RCAL-A11B|Size: 1 Each

Calibration Bath, Refrigerated, 15L, Advanced Programmable, (-30° to 200°C), 240V, 50Hz

SKU: AP15RCAL-A12E|Size: 1 Each

Chiller 1.5HP Positive Displacement Pump, Air-Cooled, 230V, 60Hz

SKU: 6860P46A270D|Size: 1 Each

Chiller 1.5HP Turbine Pump, Air-Cooled, 230V, 60Hz

SKU: 6860T56A270D|Size: 1 Each

Chiller 1.5HP Turbine Pump, Air-Cooled, 230V, 60Hz (-20° to 40°C)

SKU: 3860T56A1C0D|Size: 1 Each

Chiller 1.5HP Turbine Pump, Air-Cooled, 240V, 50Hz (-20° to 40°C)

SKU: 3850T56A1C02|Size: 1 Each

Chiller 1.5HP Turbine Pump, Water-Cooled, 230V, 60Hz

SKU: 6960T57A270D|Size: 1 Each

Chiller 1.5HP Turbine Pump, Water-Cooled, 240V, 50Hz

SKU: 6950T57A270E|Size: 1 Each

Chiller 1.5HP, Positive Displacement Pump, Air-Cooled, 240V, 50Hz

SKU: 6850P46A270E|Size: 1 Each

Chiller 1.5HP, Turbine Pump, Air-Cooled, 240V, 50Hz

SKU: 6850T56A270E|Size: 1 Each

Chiller, 1 HP, turbine pump, 240V/50Hz Cooling Capacity @ 20°C: 2.65kW Max Flow 3.5 gpm (13.2 lpm) ; Max Pressure 83 psi (5.7 bar)

SKU: CA10A2T1-41AA1N|Size: 1 System

Chiller, 1/2 HP, turbine pump, 240V/50Hz Cooling Capacity @ 20°C: 1.8kW Max Flow 3.5 gpm (13.2 lpm) ; Max Pressure 83 psi (5.7 bar)

SKU: CA05A2T1-41AA1N|Size: 1 System

Chiller, 1/3 HP, turbine pump, 240V/50Hz Cooling Capacity @ 20°C: 1.3kW Max Flow 3.5 gpm (13.2 lpm) ; Max Pressure 83 psi (5.7 bar)

SKU: CA03A2T1-41AA1N|Size: 1 System

Chiller, 1/4 HP, turbine pump, 240V/50Hz Cooling Capacity @ 20°C: 0.7kW Max Flow 3.5 gpm (13.2 lpm) ; Max Pressure 83 psi (5.7 bar)

SKU: CA02A2T1-41AA1N|Size: 1 System

Chiller, 220-240v/50Hz Cooling capacity 0.4kW @ 20°C Max flow 6.4 gpm (24.6 lpm) : max pressure 16.5psi

SKU: BA05A0G3-310A13E|Size: 1 System

Chiller, 220-240v/50Hz Cooling capacity 1.0kW @ 20°C Max flow 6.4 gpm (24.6 lpm) : max pressure 16.5psi

SKU: BA06A0G3-310A13E|Size: 1 System

Chiller, DuraChill CA, 1.0HP (2.65kW), Positive Displacement Pump, 1HP, 208-240V, 60Hz

SKU: CA10A3P2-41AA1N|Size: 1 Each

Chiller, DuraChill CA, 1.0HP (2.65kW), Turbine Pump, 1HP, 208-240V, 60Hz

SKU: CA10A3T1-41AA1N|Size: 1 Each

Chiller, DuraChill CA, 1.0HP (2.9kW), Positive Displacement Pump, 1HP, 240V, 50Hz

SKU: CA10A2P2-41AA1N|Size: 1 Each

Chiller, DuraChill CA, 1.2HP (1.74kW), Positive Displacement Pump, 1/2HP, 120V, 60Hz

SKU: CA05A1P1-41AA1N|Size: 1 Each

Chiller, DuraChill CA, 1/2HP (1.74kW) Turbine Pump, 1/2HP, 120V, 60Hz

SKU: CA05A1T1-41AA1N|Size: 1 Each

Chiller, DuraChill CA, 1/2HP (1.84kW), Positive Displacement Pump, 1/2HP, 240V, 50Hz

SKU: CA05A2P1-41AA1N|Size: 1 Each

Chiller, DuraChill CA, 1/3HP (1.28kW), Positive Displacement Pump, 1/3HP, 240V, 50Hz

SKU: CA03A2P1-41AA1N|Size: 1 Each

Chiller, DuraChill CA, 1/3HP (1.4kW), Positive Displacement Pump, 1/3HP, 120V, 60Hz

SKU: CA03A1P1-41AA1N|Size: 1 Each

Chiller, DuraChill CA, 1/3HP (1.4kW), Turbine Pump, 1/3HP, 120V, 60Hz

SKU: CA03A1T1-41AA1N|Size: 1 Each

Chiller, DuraChill CA,1/4HP (0.7kW), Positive Displacement Pump, 1/4HP, 240V, 50Hz

SKU: CA02A2P1-41AA1N|Size: 1 Each

Chiller, DuraChill CA,1/4HP (0.85kW), Positive Displacement Pump, 1/4HP, 120V, 60Hz

SKU: CA02A1P1-41AA1N|Size: 1 Each

Chiller, DuraChill CA,1/4HP (0.85kW), Turbine Pump, 1/4HP, 120V, 60Hz

SKU: CA02A1T1-41AA1N|Size: 1 Each

Coliform Bath, 28L, MX (Ambient +10° to 135°C), 120V, 60Hz

SKU: MX28C135-A11B|Size: 1 Each

Coliform Bath, 28L, MX (Ambient +10° to 135°C), 240V, 50Hz

SKU: MX28C135-A12E|Size: 1 Each

Cooling Coil Kit for Open Bath Circulators with 3.63” dia access port in bridge

SKU: 510-649|Size: 1 Each

External Bypass (for Chillers with M pump)

SKU: 510-147|Size: 1 Each

Fitting , 1/2” male NPT to M16 x 13 mm (1/2”), brass, straight, quantity 2

SKU: 775-048|Size: 1 Each

Fitting, 1/2” (13 mm) barb x 1/4” (6 mm) barb, glass filled nylon (for MX Controllers)

SKU: 776-207|Size: 1 Each

Fitting, 1/2” (13 mm) barb x 3/8” (9.5 mm) barb, glass filled nylon (for MX Controllers)

SKU: 776-205|Size: 1 Each

Fitting, 1/2” (13 mm) barb x 5/16” (5 mm) barb, glass filled nylon (for MX Controllers)

SKU: 776-206|Size: 1 Each

Fitting, 1/2” male NPT x 1/2” (13 mm) hose barb, nylon, straight

SKU: 300-096|Size: 1 Each

Fitting, 1/2” male NPT x 3/8” (9.5 mm) hose barb, brass, elbow

SKU: 775-047|Size: 1 Each

Fitting, 1/2” male NPT x 3/8” (9.5 mm) hose barb, brass, straight

SKU: 776-196|Size: 1 Each

Fitting, 1/2” male NPT x 5/8” (16 mm) hose barb, nylon, straight

SKU: 300-131|Size: 1 Each

Fitting, 1/4” male NPT x 1/4” (6 mm) hose barb, brass

SKU: 776-194|Size: 1 Each

Fitting, 1/4” male NPT x 1/4” (6 mm) hose barb, nylon

SKU: 300-048|Size: 1 Each

Fitting, 1/4” male NPT x 1/4” (6 mm) hose barb, stainless steel

SKU: 776-203|Size: 1 Each

Fitting, 1/4” male NPT x 3/16” (5 mm) hose barb, brass

SKU: 776-193|Size: 1 Each

Fitting, 1/4” male NPT x 3/16” (5 mm) hose barb, nylon

SKU: 300-049|Size: 1 Each

Fitting, 1/4” male NPT x 3/16” (5 mm) hose barb, stainless steel

SKU: 776-204|Size: 1 Each

Fitting, 1/4” male NPT x 3/8” (9.5 mm) hose barb, brass

SKU: 776-195|Size: 1 Each

Fitting, 1/4” male NPT x 3/8” (9.5 mm) hose barb, nylon

SKU: 300-047|Size: 1 Each

Fitting, 1/4” male NPT x 3/8” (9.5 mm) hose barb, stainless steel

SKU: 776-202|Size: 1 Each

Fitting, 1/4” male NPT x 5/16” (8 mm) hose barb, straight, brass, 2 each

SKU: 060305-2|Size: 1 Each

Fitting, 1/4” male NPT x M16x1, stainless steel

SKU: 775-290|Size: 1 Each

Flash Drive, PolyScience-branded, 2 GB

SKU: 250-098|Size: 1 Each

Floating Ball Cover, 3/4” (19 mm) dia., 400/package

SKU: 060301|Size: 1 Each

Flow adapter, two (2) port manifold w/shut offs, brass 1/4” male NPT x 2 @ 1/4” (6 mm) hose barb

SKU: 510-666|Size: 1 Each

Heated Circulator, 15L, Advanced Digital (Ambient +10° to 200°C), 120V, 60Hz

SKU: AD15H200-A11B|Size: 1 Each

Heated Circulator, 15L, Advanced Digital (Ambient +10° to 200°C), 240V, 50Hz

SKU: AD15H200-A12E|Size: 1 Each

Heated Circulator, 15L, Advanced Programmable (Ambient +10° to 200°C), 120V, 60Hz

SKU: AP15H200-A11B|Size: 1 Each

Heated Circulator, 15L, Advanced Programmable (Ambient +10° to 200°C), 240V, 50Hz

SKU: AP15H200-A12E|Size: 1 Each

Recent Breakthroughs

Metabolomic insights into Lyme neuroborreliosis: Exploring cerebrospinal fluid for diagnostic clues.

Kuukkanen I, Pietikäinen A, Rissanen T, Hurme S, Kortela E, Hytönen J, Karonen M.

Ticks Tick Borne Dis

PMID:42001681

Free PMC article

Lyme neuroborreliosis (LNB), a disseminated manifestation of Lyme borreliosis (LB), arises when Borrelia burgdorferi sensu lato (Bbsl) spirochetes disseminate within the host and damage the peripheral nervous system and meninges, and in rare cases, also the parenchyma of the central nervous system (CNS). While early-stage LB is diagnosed clinically, accurate diagnosis of LNB requires cerebrospinal fluid (CSF) analysis, demonstrating pleocytosis and intrathecal synthesis of Bbsl-specific antibodies. There are, however, limitations in current LNB diagnostics, such as the unspecific nature of pleocytosis and post-treatment persistence of intrathecal antibodies necessitating the search for novel biomarkers. In this study, we employed untargeted ultrahigh-performance liquid chromatography coupled with tandem mass spectrometry (UHPLC-MS/MS) to profile small metabolites (<1500 Da) in CSF samples from subjects with definite acute LNB. Comparative analyses of metabolite profiles were performed between pretreatment subject samples (n = 63) and the following groups: (A) a subset of samples collected three weeks after treatment initiation from the same individuals (n = 36), (B) Bbsl antibody-negative subjects (non-LNB, n = 61), (C) subjects with other CNS infections (n = 21). Additionally, pretreatment LNB samples were compared between individuals with radiculitis (n = 40) and those without radiculitis (n = 23) (D). Out of 4222 molecular features (MFs) detected, 131 were prioritized based on statistical significance and magnitude of change for further detailed structural characterization. Altered metabolite classes included compounds from lysophospholipids [e.g., lysophosphatidylcholine (16:0), and lysophosphatidylethanolamine (18:0)], sphingomyelins [e.g., sphingomyelin (d18:1/14:0) and sphingomyelin d16:1/16:0)], sphingoid bases (e.g., d19:0 sphinganine, and 3-ketosphingosine), fatty acid amides (e.g., palmitoleamide and oleamide), cyclic phosphatidic acids [i.e., cyclic phosphatidic acid (16:0) and cyclic phosphatidic acid (18:2)], and amino acid metabolism (i.e., DL-glutamine, 5-hydroxytryptophan and DL-kynurenine). These findings underscore the potential of CSF metabolomics as a powerful complementary tool for diagnosing LNB and differentiating it from other CNS conditions. The identified metabolic signatures offer a foundation for future biomarker development and may enhance diagnostic precision, guide treatment strategies, and deepen our understanding of LNB pathogenesis.

Isotope Dilution NanoLC-MS/MS Quantitation of Methylglyoxal DNA-Protein Cross-Links: Formation and Repair in Human Cells.

Omondi RO, Barnes EM, Gurajala KC, Fisette G, Chaudray IA, Erber L.

Anal Chem

PMID:41995195

Free PMC article

DNA-protein cross-links (DPCs) represent a prevalent form of DNA damage that forms when cellular proteins become covalently trapped to DNA strands upon exposure to various endogenous and exogenous agents. Methylglyoxal is an endogenous metabolite that reacts with guanine and adenine bases in DNA and RNA, as well as cysteine, arginine, and lysine residues in proteins, generating advanced glycation end-products (AGEs), including DPCs. These modifications have been linked to human disease, including cancer, liver disease, diabetes, and neurodegenerative disorders. Herein, we present a mass spectrometry method for quantifying MGO-induced DNA-protein cross-links (DPCs) in human cells. We prepared an isotope 15 N 2 13 C 6 -dG-MGO-Lys internal standard and developed a quantitative liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for detecting and quantifying the formation and repair of dG-MGO-Lys DPCs in cells. Genomic DNA was extracted, subjected to sequential protease and nuclease digestion, purified by offline high-performance liquid chromatography (HPLC), and analyzed by LC-MS/MS. The method's standard curve showed a strong linear relationship across a concentration range of 10-1000 fmol ( R 2 = 0.9994). The method achieved limits of detection (LOD) and quantification (LOQ) of 10 and 20 fmol, respectively. Inhibition of proteasome and SPRTN activity revealed that SPRTN functions as a predominant proteolytic enzyme in MGO DPC repair. Overall, this analytical approach can offer valuable insights into the relevance of DPCs in diseases linked to elevated MGO levels.

Label-Free High-Throughput Screening of CYP3A4 Inhibitors Using Acoustic Ejection Mass Spectrometry.

Rimmer MA, Wang J, Ranathunge TA, Shi Z, Li Y, Lee D, Chai SC, Lin W, Twarog NR, Ansari AZ, Shelat AA, Young BM, Chen T, Yang L.

Anal Chem

PMID:41997578

Free PMC article

Label-free high-throughput screening (HTS) technologies have greatly accelerated early drug discovery by allowing direct measurement of biochemical activity without the need for chemical labels. Techniques like MALDI-TOF, RapidFire, desorption electrospray ionization, and acoustic-mist ionization mass spectrometry have shown strong analytical performance. More recently, acoustic droplet ejection mass spectrometry (AEMS) has emerged as a next-generation platform capable of analyzing over a thousand data points per hour. In this study, we thoroughly evaluated an AEMS workflow using cytochrome P450 3A4 (CYP3A4) as a model enzyme. Screening a library of 9702 compounds, this work represents the first successful label-free HTS campaign for CYP3A4 inhibitors in a 384-well format using AEMS. The result using nifedipine as a substrate was comparable to the widely used P450-Glo luminescence assay but with fewer inhibitor hits identifications. To further compare and parse differences between the methods and the substrates, 819 hits were retested using AEMS with luciferin isopropyl acetal, the same substrate used in the luminescence assay. The findings confirmed that both the assay method and the substrate influence which inhibitors were detected. Overall, this study established AEMS as a robust and reliable tool for CYP3A4 inhibitor screening. It offers high analytical precision, fewer false positives, compatibility with multiple substrates, and direct detection of metabolites, providing a practical way to validate hits and improve confidence in early drug discovery efforts.

Quantification of the Active Metabolites 4-Methylaminoantipyrine and 4-Aminoantipyrine of Dipyrone From Human Plasma by LC-MS/MS.

Schmidt S, Ihmsen H, Golditz T, Schüttler J, Wehrfritz A.

Int J Anal Chem

PMID:42004449

Free PMC article

Dipyrone (metamizole) is widely used as a nonopioid analgesic in perioperative and intensive care settings; however, its administration may be associated with severe adverse effects. In this study, we describe an analytical assay for the quantification of the active metabolites 4-methylaminoantipyrine (4-MAA) and 4-aminoantipyrine (4-AA) in human plasma using ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS). In addition, the protein-unbound fractions of both metabolites were determined by ultrafiltration. Chromatographic separation was performed on a UPLC system using gradient elution, followed by MS/MS detection with an electrospray ionization source. The limits of detection for both metabolites were 100 ng/mL. Across the investigated concentration range (100-10,000 ng/mL), the relative error (%RE) ranged from -6.3% to +3.5%. Intra-day and inter-assay variability were below 10%. Method validation was conducted in accordance with the 2018 FDA Bioanalytical Method Validation Guidance, and all evaluated parameters met the required criteria for analytical accuracy and precision. The developed assay is suitable for monitoring dipyrone metabolites and may support the prevention of potential overdosing during prolonged analgesic therapy or in intensive care settings.

Metabolomics profiling of acute myelogenous leukemia patients to identify systemic differences associated with in vitro sensitivity to SYK inhibitors.

Brattås MK, Hatfield KJ, Paulsen Rye K, Reikvam H.

Metabolomics

PMID:41995902

Free PMC article

Introduction Acute myelogenous leukemia (AML) is a heterogeneous hematological malignancy. Despite therapeutic advances, high relapse rates and poor survival persist, underscoring the need for new treatment strategies. Spleen tyrosine kinase (SYK) is crucial for leukemic cell survival and proliferation, and SYK inhibition is being explored in AML therapy. Objective This study aimed to identify whether distinct baseline serum metabolic profiles can differentiate between AML patient samples exhibiting high versus low in vitro sensitivity to SYK inhibition. Methods Leukemic cells from 49 AML patients were evaluated for their in vitro antiproliferative response to SYK-selective inhibitors (entospletinib, RO9021) and dual SYK/FLT3 inhibitors (fostamatinib, TAK-659). Serum samples from the same patients were subjected to untargeted metabolomic profiling using liquid chromatography-mass spectrometry (LC-MS). Bioinformatic analyses were applied to identify metabolic signatures associated with differential drug sensitivity. Results Hierarchical clustering identified three drug sensitivity groups, i.e. high, intermediate, low. Untargeted metabolomics identified 1204 metabolites across major pathways. Comparative analyses revealed distinct metabolic differences between patients with high versus low sensitivity to SYK inhibitors. For dual SYK/FLT3 inhibitors, lipid metabolites consistently were more discriminative for drug response, while for SYK selective inhibitors the profiles were more heterogeneous. Pathway analyses highlighted alterations in amino acid, lipid, and xenobiotic metabolism, including arginine biosynthesis and glycine/serine metabolism, as potential determinators for effect of SYK inhibition. Conclusion This study highlights potential metabolite signatures associated with in vitro SYK-targeted therapy in AML. These findings support future research to identify potential serum biomarkers, guiding personalized SYK inhibition strategies in AML.