Macrophages (human) - Clone 25F9, FITC labeled
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Catalog number
T-1018.0200
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Price
Please ask
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Size
200ug
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StorageTemperature
+5 ± 3 °C
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HOST
Mouse
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Clone
25F9-FITC
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Format
Purified, lyophilized
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Species Crossreactivity
human
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Application
IHC, FACS
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Conjugate
NA
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Top Categories
Antibodies
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Keywords
Monoclonal; primary antibody
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Additonal Description
Application: IHC (f)
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Properties
This Penlabs Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Penlabs FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
Anti-FITC Antibody
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Description
FITC labelling for flow cytometry of antibodies and peptides.
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Gene target
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Short name
Macrophages ( ) - Clone 25F9, FITC
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Technique
FITC, Fluorescein
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Isotype
IgG1
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Label
FITC
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Species
Human, Humans
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Alternative name
Macrophages (H. sapiens) - clonality 25F9, fluorecein labeled
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Alternative technique
fluorescine
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Clone name
25F9
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MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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