Recombinant Human E3 Ubiquitin-Protein Ligase CHIP/CHIP

• Catalog number
  C115-10
• Price
  Please ask
• Size
  10 ug

• Description
  Recombinant Human STUB1 is produced by our E.coli expression system and the target gene encoding Met1-Tyr303 is expressed.
• Species reactivity
  Human
• Origin
  Escherichia coli
• Peptide sequence
  MKGKEEKEGGARLGAGGGSPEKSPSAQELKEQGNRLFVGRKYPEAAACYGRAITRNPLVAVYYTNRALCYLKMQQHEQALADCRRALELDGQSVKAHFFLGQCQLEMESYDEAIANLQRAYSLAKEQRLNFGDDIPSALRIAKKKRWNSIEERRIHQESELHSYLSRLIAAERERELEECQRNHEGDEDDSHVRAQQACIEAKHDKYMADMDELFSQVDEKRKKRDIPDYLCGKISFELMREPCITPSGITYDRKDIEEHLQRVGHFDPVTRSPLTQEQLIPNLAMKEVIDAFISENGWVEDY
• Estimated molecular weight
  34,86 kDa
• Protein purity
  Greater than 95% as determined by reducing SDS-PAGE.
• Endotoxin level
  Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test.
• Shipping condition
  Dry ice/ice packs
• Package form
  Supplied as a 0.2 µm filtered solution of 20mM PB, 150mM NaCl, pH 7.2.
• Storage conditions
  Store at below -20°C, stable for 6 months after receipt. Please minimize freeze-thaw cycles.
• Reconstitution conditions
  Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100 µg/ml.Dissolve the lyophilized protein in ddH2O.Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• UniProt number
  Q9UNE7
• Other related products
• Properties
  Precipitation of Chromatins is a pull down technique of native DNA sequences by specific binding proteins on which on the other side of the protein the antibody can bind. Lots of antibodies are not suited for Chip analysis pulldown because they bind on the binding site of the DNA. The novo antibody is proven to not bind on the DNA binding site and is suitable for ChiP precipitation supplied in 1 vials. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Ubiquitin-Protein   Ligase  
• Gene symbol
  ITCH, HECTD3, PRKN
• Short name
  Recombinant E3 Ubiquitin-Protein Ligase CHIP/CHIP
• Technique
  Recombinant, ChiP, Chromatine immuno Precipitationg antibody, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. novo advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Human STUB1/CHIP
• Alternative technique
  rec, epigenetics

Gene info

• Identity
  HGNC:13890
• Gene
  ITCH
• Long gene name
  itchy E3 ubiquitin protein ligase
• Synonyms gene name
  • itchy (mouse homolog) E3 ubiquitin protein ligase
  • itchy E3 ubiquitin protein ligase homolog (mouse)
• Synonyms
  • AIP4
• GenBank acession
  • AF095745
• Locus
  20q11.22
• Discovery year
  2001-04-27
• Entrez gene record
  83737
• Pubmed identfication
  • 11318614
• Classification
  • C2 domain containing
  • HECT domain containing
  • MicroRNA protein coding host genes
• VEGA ID
  OTTHUMG00000032300
• Locus Specific Databases
  • LRG_354

Gene info

• Identity
  HGNC:26117
• Gene
  HECTD3
• Long gene name
  HECT domain E3 ubiquitin protein ligase 3
• Synonyms gene name
  • HECT domain containing E3 ubiquitin protein ligase 3
• Synonyms
  • FLJ21156
• GenBank acession
  • BC019105
• Locus
  1p34.1
• Discovery year
  2005-07-07
• Entrez gene record
  79654
• Pubmed identfication
  • 18194665
• RefSeq identity
  • NM_024602
• Classification
  • HECT domain containing
• VEGA ID
  OTTHUMG00000008587

Gene info

• Identity
  HGNC:8607
• Gene
  PRKN
• Long gene name
  parkin RBR E3 ubiquitin protein ligase
• Synonyms gene
  • PARK2
• Synonyms gene name
  • Parkinson disease (autosomal recessive, juvenile) 2, parkin
  • parkinson protein 2, E3 ubiquitin protein ligase (parkin)
• Synonyms
  • PDJ
  • AR-JP
  • parkin
• Synonyms name
  • E3 ubiquitin ligase
• Locus
  6q26
• Discovery year
  1997-08-18
• Entrez gene record
  5071
• Pubmed identfication
  • 9560156
  • 9570960
• Classification
  • RBR E3 ubiquitin ligases
• VEGA ID
  OTTHUMG00000015970
• Locus Specific Databases
  • Global Variome shared LOVD

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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