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Category
Primary Antibodies
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Long description
Members in the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-1 (for TNF- and ApoL-related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NF- B and c-jun N-terminal Kinase) (1-4). BAFF/BLyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion (1,2). Two receptors for BAFF were recently identified and designated TACI and BCMA (5). BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases (5,6). Like TNF and TRAIL, THANK was shown to activate NF- B and c-jun N-terminal kinase (JNK) and to induce apoptosis (4).
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Antibody come from
Synthetic peptide corresponding to amino acids 254 to 269 of the human BAFF protein.
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Other description
Provided in phosphate buffered saline solution containing 0.02% sodium azide as a preservative. Antigen Immunoaffiinity Purification
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti Tumor necrosis factor ligand superfamily member 13B (C Terminal) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human, Mouse, Rat
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
Detects BAFF by Western blot at 0.25 to 1 µg/ml. Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Moore, P.A., et al., BLyS: member of the tumor necrosis factor family and B lymphocyte stimulator. Science 1999, 285, 260-263_x000B__x000B_2. Schneider, P., et al. BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth. J. Exp. Med. 1999, 189, 1747-1756_x000B__x000B_3. Shu, H.B., et al., TALL-1 is a novel member of the TNF family that is down-regulated by mitogens. J. Leukoc. Biol. 1999, 65, 680-683_x000B__x000B_4. Mukhopadhyay, A., et al., Identification and characterization of a novel cytokine, THANK, a TNF homologue that activates apoptosis, nuclear factor-kappaB, and c-Jun NH2-terminal kinase. J. Biol. Chem. 1999, 274, 15978-15981_x000B__x000B_5. Gross, J.A., et al., TACI and BCMA are receptors for a TNF homologue implicated in B-cell autoimmune disease. Nature 2000, 404, 995-999_x000B__x000B_6. Khare, S.D., et al. Severe B cell hyperplasia and autoimmune disease in TALL-1 transgenic mice. Proc. Natl. Acad. Sci. USA 2000, 97, 3370-3375
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells. FAS ligand and other ligands are binding to the receptor for signaling pathways for example in apoptosis or JNK signaling. Receptor agonists are often tested for drug development.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
