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Category
Secondary Antibodies
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Long description
The reactivity of the antiserum is directed to the heavy chains of immunoglobulin isotypes IgG, IgA, IgM, IgD and IgE. The antiserum contains antibodies to common determinants and to the surface determinants of the common Fab as tested by immunoelectrophoresis and double radial immunodiffusion. It does not react with any non-Ig proteins in mouse serum. In immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of immunoglobulins in mouse serum or other body fluids; to prepare an immunoadsorbent for the purification of mouse immunoglobulins from serum or plasma; as secondary antibody to precipitate the immunoglobulin in normal mouse serum. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such use labelled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
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Antibody come from
Purified polyclonal mouse IgG and pools of homogenous IgG, IgA, IgM, IgD and IgE. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Other description
Delipidated, heat inactivated, lyophilized stable whole serum. No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled rabbit serum. No foreign proteins added.
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti Mouse IgG1 IgG2a IgG2b IgG3 IgA IgM IgD IgE (heavy and light chains) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Mouse
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Antibody s reacts with these species
Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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Antibody s specificity
Precipitating polyclonal Rabbit antiSerum to Mouse immunoglobulins IgG, IgA, IgM, IgD and IgE, heavy and light chains
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Research interest
Veterinary
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Application
Immunoprecipitation
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Antibody s suited for
Immunoprecipitation. In immunoelectrophoresis use 2 μl or equivalent against 120 μl antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and diluted) in 2 mm diameter peripheral wells. Precipitin titre not less than 1:32 when tested against normal mouse serum in agar immunodiffusion block titration.
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Storage
The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Lyophilized at +4°C--at least 10 years. Reconstituted at or below -20°C--3-5 years. Reconstituted at +4°C--7 days
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Relevant references
no information yet
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. This is against a protein of mus musculus directed antibody.
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Properties
Immunoglobulin A (IgA, also referred to as sIgA) is an antibody that plays a critical role in immune function in the mucous membranes. More IgA is produced in mucosal linings than all other types of antibody combined. Examples are EMA, ACA ELISA test kits and for diagnostic detection the IgA of Anti-Beta-2 Glycoprotein 1; β2-Glycoprotein 1 Antibodies; Beta 2GP1 Ab, Beta-2 Glycoprotein 1 Antibodies IgG, IgM, and IgAs. Immunoglobulin D (IgD) is an antibody isotype that makes up about 1% of proteins in the plasma membranes of mature B-lymphocytes where it is usually coexpressed with another cell surface antibody called IgM. IgD is also produced in a secreted form that is found in very small amounts in blood serum, representing 0.25% of immunoglobulins in serum for virus detection. Anti-viral immunoglobulin D antibodies have a diagnostic value. Immunoglobulin E (IgE) kappa or Fc specific antibody is a kind of antibody (or immunoglobulin (Ig) "isotype") that has only been found in mammals. IgE is synthesized by plasma cells. Monomers of IgE consist of two heavy chains (ε chain) and two light chains, with the ε chain containing 4 Ig-like constant domains (Cε1-Cε4). IgEs play a role in allergy and response to parasite infection. High levels of IgEs caused by parasites can lower the allergic reaction of patients to allergens common present in the human environment. As such the parasite history of an allergic patient needs to be taken in consideration as a positive factor. Immunoglobulin M, or IgM for short, is a basic antibody that is produced by B cells. IgM is by far the physically largest antibody in the human circulatory system. It is the first antibody to appear in response to initial exposure to an antigen
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Oryctolagus cuniculus
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Gene target
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Short name
Rabbit anti Mouse IgG1 IgG2a IgG2b IgG3 IgA IgM IgD IgE (heavy light chains)
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Technique
anti mouse, Rabbit, light, Mouse, anti, IgA, IgD, IgE, IgM, antibody to, anti mouse antibodies, IgAs, IgDs, IgEs, IgMs, mouses
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Host
Rabbit, Rabbits
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Isotype
not specified, IgA, IgD, IgE, IgG1, IgG2a, IgG2b, IgG3, IgM
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Label
unlabeled
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Species
Mouse, Mouses
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Alternative name
production species: rabbit antibody to Mouse IgG1 IgG2a IgG2b IgG3 Immunoglobulin A Immunoglobulin M IgD immunoglobuline E (heavy and light chains)
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Alternative technique
rabbit-anti, murine, antibodies, immunoglobulin-a, immunoglobulin-d, immunoglobulin-e, immunoglobulin-m
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