Rabbit anti Chicken ovalbumin

  • Catalog number
    RACh/Oalb
  • Price
    Please ask
  • Size
    1 mL
  • Category
    Primary Antibodies
  • Long description
    A single precipitin line is obtained in immunoelectrophoresis against 1% chicken egg albumin in PBS. In double radial immunodiffusion of the antiserum against twofold dilutions of 1% chicken egg albumin in PBS and in the reverse arrangement of the chicken egg albumin solution against twofold dilutions of the antiserum, a singe precipitin line is obtained. The antiserum does not react with chicken serum. In precipitating techniques as immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify the presence of chicken ovalbumin or to determine its concentration. The presence of non-precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in non-precipitating antibodybinding techniques as ELISA and Western blotting if proper controls are included.
  • Antibody come from
    Highly purified egg albumin (MW 45,000) isolated from chicken egg whites. Freund’s complete adjuvant is used in the first step of the immunization procedure.
  • Other description
    Delipidated, heat inactivated, lyophilized, stable whole serum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. No foreign proteins added.
  • Clone
    Polyclonal
  • Antigen antibody binding interaction
    Rabbit anti Chicken ovalbumin Antibody
  • Antibody is raised in
    Rabbit
  • Antibody s reacts with
    Chicken
  • Antibody s reacts with these species
    Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
  • Antibody s specificity
    Precipitating polyclonal Rabbit antiSerum to Chicken ovalbumin.
  • Research interest
    Veterinary
  • Application
    Immunoprecipitation
  • Antibody s suited for
    Immunoprecipitation. Precipitin titre not less than 1:16 when tested against an 1% solution of chicken egg albumin in agar-block immunodiffusion titration.
  • Storage
    The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days.
  • Relevant references
    no information yet
  • Protein number
    see ncbi
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
  • Properties
    Chickens like all bird species have IgY antibodies that are very stable and found in the yolk. Gallus Gallus domestica is the Latin name of the Chicken.
  • About
    Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
  • Gene symbol
    SERPINB10, SERPINB8P1
  • Short name
    Rabbit anti ovalbumin
  • Technique
    Rabbit, anti, antibody to
  • Host
    Rabbit, Rabbits
  • Isotype
    not specified
  • Label
    unlabeled
  • Species
    chicken, Chickens
  • Alternative name
    production species: rabbit antibody to Chicken ovalbumin
  • Alternative technique
    rabbit-anti, antibodies
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    serpin family B member 8 pseudogene 1
  • Synonyms gene
  • Synonyms gene name
    • serine (or cysteine) proteinase inhibitor, clade B (ovalbumin) pseudogene 1
    • serpin peptidase inhibitor, clade B (ovalbumin) pseudogene 1
    • serpin peptidase inhibitor, clade B8 (ovalbumin) pseudogene 1
  • Locus
  • Discovery year
    1999-08-20
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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