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Category
Primary Antibodies
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Long description
CERT mediates the ATP-dependent ER-to-Golgi transfer of ceramide in a non-vesicular manner. The biosynthesis of lipids involves steps that occur in different intracellular compartments. The movement of lipids within these compartments is important in lipid-mediated signalling. Human CERT is identical to a splice variant of human Goodpasture antigen-binding protein (GPBP26)._x000B__x000B__x000B_CERT contains a phosphoinositide-binding pleckstrin-homology (PH) domain (which targets CERT to the Golgi by binding phosphatidylinositol-4-phosphate (PtdIns4P)), a middle region, and a putative lipid-transfer-catalysing domain called START. CERT and CERTL can specifically extract ceramide from phospholipid bilayers in a START-domain-dependent manner. CERT interacts with ER membranes and specifically extracts ceramide. CERT catalyses both the specific extraction of ceramide from donor vesicles and its transfer to acceptor vesicles. CERT can associate with the Golgi in a PtdIns4P dependent manner. _x000B_
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Antibody come from
Synthetic protein made to the N-terminal region of human DDR1
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Other description
Phosphate buffered saline containing 0.1% sodium azide. Ammonium Sulfate Precipitation
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Clone
Polyclonal
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Antigen antibody binding interaction
Rabbit anti Ceramide Transfer Protein (CERTL) Antibody
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Antibody is raised in
Rabbit
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Western Blot
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Antibody s suited for
Antibody can be used for immunohistochemistry (15-20 µg/ml, formalin-fixed paraffin-embedded tissues). Optimal concentration should be evaluated by serial dilutions.
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Storage
-20ºC
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Relevant references
1. Xu, H., et al. 'Collagen binding specificity of the discoidin domain receptors: binding sites on collagens II and III and molecular determinants for collagen IV recognition by DDR1.' Matrix Biol. 2011, 30, 16-26_x000B__x000B_2. Eswaramoorthy, R., et al. 'DDR1 regulates the stabilization of cell surface E-cadherin and E-cadherin-mediated cell aggregation.' J. Cell Physiol. 2010, 224, 387-397_x000B__x000B_3. Huang, Y., et al. 'The collagen receptor DDR1 regulates cell spreading and motility by associating with myosin IIA.' J. Cell Sci. 2009, 122, 1637-1646
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
