-
Category
Primary Antibodies
-
Long description
Sphingolipids and their metabolic products, including ceramide, sphingosine, and sphingosine-1-phosphate (S1P),1 are important signaling molecules in many biological processes. Ceramide has been is one of the key elements regulating apoptotic responses. Ceramide has been reported as a regulator of several protein kinases and phosphatases, including ceramide-activated protein kinase, protein phosphatase, and protein kinase C. Sphingosine, a metabolite of ceramide, might also play a role in mitochondria-mediated apoptosis. Sphingosine inhibits several protein kinases, including protein kinase C and Ca2 -calmodulin-dependent kinase II. S1P regulates many biological processes, including mitogenesis, differentiation, migration, and suppression of apoptosis.
-
Antibody come from
Hybridoma produced by the fusion of splenocytes from BALB/c mice immunized with a cytokeratins from the human bladder carcinoma cell line T24 and SP2/0-Ag14 mouse myeloma cells.
-
Other description
Provided as solution in phosphate buffered saline with 0.08% sodium azide.
-
Clone
Polyclonal
-
Antigen antibody binding interaction
Rabbit anti Ceramide Kinase (rProtein-Full Length) Antibody
-
Antibody is raised in
Rabbit
-
Antibody s reacts with
Human
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
No Data Available
-
Application
ELISA, Western Blot
-
Antibody s suited for
Antibody can be used for immunoblotting (1:100-1:1000), immunocytochemistry, immunohistochemistry on frozen sections and flow cytometry (1:100-1:200).Optimal concentration should be evaluated by serial dilutions.
-
Storage
-20ºC
-
Relevant references
1. Ramaekers, F., et al. (1987). Tissue distribution of keratin 7 as monitored by a monoclonal antibody, Exp Cell Res 170, 235-49._x000B__x000B_2. Smedts, F., et al. (1990). Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia, Am J Pathol 136, 657-68._x000B__x000B_3. Schaafsma, H. E., et al. (1990). Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression, Am J Pathol 136, 329-43._x000B__x000B_4. Ramaekers, F., et al. (1990). Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas, Am J Pathol 136, 641-55._x000B__x000B_5. Bauwens, L. J., et al. (1992). Expression of intermediate filament proteins in the adult human vestibular labyrinth, Ann Otol Rhinol Laryngol 101, 479-86._x000B__x000B_6. Smedts, F., et al. (1992). Keratin expression in cervical cancer, Am J Pathol 141, 497-511._x000B__x000B_7. Ivanyi, D., et al. (1992). Patterns of expression of feline cytokeratins in healthy epithelia and mammary carcinoma cells, Am J Vet Res 53, 304-14.
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
About
Rabbits are used for polyclonal antibody production by nordc. Rabbit antibodies are very stable and can be stored for several days at room temperature. nordc adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
-
Latin name
Oryctolagus cuniculus
