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Category
Proteins & Peptides
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Long description
Sphingosine kinase 1 catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (spp)(sphingosine + ATP = sphingosine 1-phosphate + ADP), a lipid mediator with both intra- and extracellular functions. also acts on d-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as d,l-threo-dihydrosphingosine, n,n-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol. The long form of sphingosine kinase 1, an N-terminal extension, is responsible for FceRI-mediated calcium mobilization and may play a role in other ligand mediated signaling events.
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Antibody come from
Purified recombinant human EGF.
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Other description
Purified IgG in phosphate buffered saline, pH 7.4 containing 0.1% azide.
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Clone
not specified
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Antigen antibody binding interaction
Phospho Sphingosine Kinase 1 (Ser225) antibody Mouse - Blocking Peptide
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Antibody is raised in
see techfile
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Antibody s reacts with
see techfile
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Application
Antibody function blocking
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Antibody s suited for
Immediately prior to use, dilute to a concentration of 1-2.5 µg/mL in an appropriate coating buffer. Coat each well with 100 µL. Alternatively, the antibody may be diluted in a buffered solution with carrier protein, such as phosphate buffered saline with 1% BSA. .Optimal concentration should be evaluated by serial dilutions. Suitable for use in ELISA as a capture antibody.
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Storage
-20ºC
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Relevant references
1. Baiula, M., et al. ÔREST is up-regulated by epidermal growth factor in HeLa cells and inhibits apoptosis by influencing histone H3 acetylation.Õ Biochim Biophys Acta, 1823, 1252-1263 (2012)_x000B__x000B_2. Barisic, K., et al. ÔRegulation of Na+/H+ exchanger by urogastrone, a potent activator of cell proliferation.Õ 51, 483-491 (2002)
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. blocking peptide
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Test
You can block the antibody by the specific target amino acid sequence of peptide. Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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Description
Peptides short amino acid chains or epitopes or blocking antagonists. The shortest peptides are dipeptides, consisting of 2 amino acids joined by a single peptide bond, followed by tripeptides, tetra peptides, ... till polypeptides that are long, continuous, and unbranched synthetic peptide chains. These biological oligomers and polymers can be Solid-phase peptide synthesis (SPPS), or in continue produced for custom peptide synthesis projects. The High-efficiency solid phase peptide synthesis (HE-SPPS) is give very low production costs.
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French translation
anticorps
