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Category
Primary Antibodies
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Long description
Vimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP, often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type.
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Antibody come from
V9 is a Mouse monoclonal IgG1 antibody derived by fusion of PAI Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with vimentin isolated from porcine lens.
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Other description
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
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Clone
V9
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Antigen antibody binding interaction
Mouse anti Vimentin Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Chicken,Human,Rat,Sheep
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Antibody s reacts with these species
Also reacts with potoroo
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Antibody s specificity
V9 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
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Research interest
Cancer,Cytoskeleton,Immunology,Neurobiology,Stem cell research
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Application
Flow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
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Antibody s suited for
V9 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. For staining paraffin-embedded tissues pretreatment by boiling tissue sections for 10 minutues in 10 mM citRate buffer (pH 6.0) is required. Optimal antibody dilution should be determined by titration; recommended range is 1:25 – 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:1000 for immunoblotting applications.
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Storage
Store at 4°C, or in small aliquots at -20°C.
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Relevant references
Osborn, M., Debus, E., and Weber, K. (1984). Monoclonal antibodies specific for vimentin, Eur J Cell Biol 34, 137-43. Tolle, H. G., Weber, K., and Osborn, M. (1985). Microinjection of monoclonal antibodies specific for one intermediate filament protein in cells containing multiple Keratins allow insight into the composition of particular 10 nm filaments, Eur J Cell Biol 38, 234-44. Van Muijen, G. N., Ruiter, D. J., and Warnaar, S. O. (1987). Coexpression of intermediate filament polypeptides in Human fetal and adult tissues, Lab Invest 57, 359-69. Van Muijen, G. N., Warnaar, S. O., and Ponec, M. (1987). Differentiation-related changes of Cytokeratin expression in cultured Keratinocytes and in fetal, newborn, and adult epidermis, Exp Cell Res 171, 331-45. Corver, W. E., Koopman, L. A., van der Aa, J., Regensburg, M., Fleuren, G. J., and Cornelisse, C. J. (2000). Four-color multiparameter DNA flow cytometric method to study phenotypic intRatumor heterogeneity in cervical cancer, Cytometry 39, 96-107. De Visscher, G., Plusquin, R., Mesure, L., Flameng, W. (2010). Selection of an immunohistochemical panel for cardiovascular research in Sheep. Appl Immunohistochem Mol Morphol 18, 382-91.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
