Mouse anti Vimentin
-
Catalog numberMUB1901S
-
PricePlease ask
-
Size1 mL
-
-
CategoryPrimary Antibodies
-
Long descriptionVimentin (57 kDa) is the intermediate filament protein (IFP) of mesenchymal cells. This IFP however often deviates from the tissue-specific and developmentally regulated pattern of expression. Besides its typical expression in most cultured cells, vimentin is also expressed together with several other IFPs during early stages of development. As differentiation proceeds, vimentin is exchanged for the tissue-specific intermediate filament type. Also in cancers, vimentin is often expressed in addition to the tissue-specific IFP.
-
Antibody come fromRV203 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a cytoskeletal vimentin extract of calf lens.
-
Other descriptionEach vial contains 1ml of culture supernatant of monoclonal antibody containing 0.09% sodium azide.
-
CloneRV203
-
Antigen antibody binding interactionMouse anti Vimentin Antibody
-
Antibody is raised inMouse
-
Antibody s reacts withCanine,Caprine,Hamster,Human,Rat,Swine
-
Antibody s reacts with these speciesThis antibody doesn't cross react with other species
-
Antibody s specificityRV203 reacts exclusively with vimentin, which is expressed in mesenchymal cells and mesenchymal derived tumors e.g. lymphoma, sarcoma and melanoma.
-
Research interestCancer,Cytoskeleton,Immunology,Neurobiology,Stem cell research
-
ApplicationFlow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
-
Antibody s suited forRV203 is suitable for immunoblotting, immunocytochemistry, immunohistochemistry on frozen and paraffin-embedded tissues and flow cytometry. Optimal antibody dilution should be determined by titration; recommended range is 1:5 – 1:10 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:5 – 1:100 for immunoblotting applications.
-
StorageStore at 4°C, or in small aliquots at -20°C.
-
Relevant referencesSchaart, G., Pieper, F. R., Kuijpers, H. J., Bloemendal, H., and Ramaekers, F. C. (1991). Baby Hamster kidney (BHK-21/C13) cells can express striated muscle type proteins, Differentiation 46, 105-15.
-
Protein numbersee ncbi
-
WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
-
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin nameMus musculus
-
Gene target
-
Gene symbolVIM2P, VIM, VMAC
-
Short nameMouse anti Vimentin
-
TechniqueMouse, anti, antibody to, mouses
-
Hostmouse
-
IsotypeIgG1
-
Labelunlabeled
-
SpeciesMouse, Mouses
-
Alternative nameMouse antibody to Vimentin
-
Alternative techniquemurine, antibodies
-
Gene info
-
Identity
-
Gene
-
Long gene namevimentin 2, pseudogene
-
Synonyms gene
-
Synonyms gene name
- vimentin pseudogene 1
- vimentin 2, pseudogene (functional)
-
Synonyms
-
Synonyms name
-
Locus
-
Discovery year2012-04-23
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namevimentin
-
GenBank acession
-
Locus
-
Discovery year2001-06-22
-
Entrez gene record
-
RefSeq identity
-
Classification
- Intermediate filaments Type III
-
VEGA ID
Gene info
-
Identity
-
Gene
-
Long gene namevimentin type intermediate filament associated coiled-coil protein
-
GenBank acession
-
Locus
-
Discovery year2009-06-12
-
Entrez gene record
-
Pubmed identfication
-
RefSeq identity
-
VEGA ID
MeSH Data
-
Name
-
ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
-
Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
-
Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data