-
Category
Primary Antibodies
-
Long description
BMI1 polycomb ring finger oncogene, also known as BMI1, is a protein which in Humans is encoded by the BMI1 gene. BMI1 (B lymphoma Mo-MLV insertion region 1 homolog) has been reported as an oncogene by regulating p16 and p19, which are cell cycle inhibitor genes. BMI1 is rapidly recruited to sites of DNA damage and it sustains for over than 8h. Loss of BMI1 leads to radiation sensitive and impaired repair of DNA double-strand breaks by homologous recombination. Bmi1 is necessary for efficient self-renewing cell divisions of adult hematopoietic stem cells as well as adult peripheral and central nervous system neural stem cells. Bmi1 is also thought to inhibit ageing in neurons through the suppression of p53. In primary and tumor cells, nuclear BMI1 shows a fine-grain distribution over chromatin, usually dense in interphase nuclei and significantly weaker along mitotic chromosomes. In addition, BMI1 preferentially associates with several distinct heterochromatic domains in tumor cell lines. Bmi1 seems to play an important role in several types of cancer, such as bladder, skin, prostate, breast, ovarian, colorectal as well as hematological malignancies. Its amplifiCation and overexpression is especially pronounced in mantle cell lymphomas. In both primary and tumor cell lines a marked cell cycle regulation of Pc-G-chromatin interaction is observed: nuclear BMI1-staining dissipates in late S phase and is reestablished early in G1-phase. Chromatin-association of BMI1 inversely correlates with its phosphorylation status in a cell cycle-dependent fashion: at G1/S, hypophosphorylated. BMI1 is specifically retained in the chromatin-associated nuclear protein fraction, whereas during G2/M, phosphorylated BMI1 is not chromatin bound.
-
Antibody come from
229F6 is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with recombinant Bmi1 protein corresponding to residues 1-202 of Mouse Bmi-1 acterial fusion protein of membrane proximal non repeat domain of Human episialin.
-
Other description
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
-
Clone
229F6
-
Antigen antibody binding interaction
Mouse anti Human BMI1 Antibody
-
Antibody is raised in
Mouse (Balb/c)
-
Antibody s reacts with
Human,Mouse,Rabbit,Rat
-
Antibody s reacts with these species
This antibody doesn't cross react with other species
-
Antibody s specificity
229F6 preferentially associates with several distinct heterochromatic domains in tumor cell lines. In both primary and tumor cell lines a marked cell cycleregulation of Pc-G-chromatin interaction is observed.
-
Research interest
Cancer, Stem cell research
-
Application
Immunohistochemistry (frozen),Immunoprecipitation,Western blotting
-
Antibody s suited for
229F6 is useful for immunoprecipitation, western blot and immunohistochemistry on frozen sections. Optimal antibody dilution should be determined by titration.
-
Storage
Store at 4°C, or in small aliquots at –20°C.
-
Relevant references
Jan Willem Voncken, Dieter Schweizer, Louise Aagaard, Lydia Sattle, Michael F. Jantsch and Maarten van Lohuizen. Chromatin-association of the Polycomb group protein BMI1 is cell cycle regulatedand correlates with its phosphorylation status. J. of Cell Science 112: 4627-4639, 1999. Mark J. Alkema, Marieke Bronk, Els Verhoeven, Arie Otte, Laura J. van 't Veer, Anton Berns and Maarten van Lohuizen. IdentifiCation of BMI1-interacting proteins as constituents of a multimeric mammalian Polycomb complex. Genes Dev. 11: 226-240, 1997.
-
Protein number
see ncbi
-
Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
-
-
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
-
Properties
Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
-
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
-
Latin name
Mus musculus
