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Category
Primary Antibodies
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Long description
Identification of CD15 that recognizes a human mylelomonocytic antigen. The structure recognized by CD15 antibodies is lacto-N-fucopentose III.1. The CD15 antigen is present on greater than 95% of mature peripheral blood eosinophils and neutrophils. It is present at low density on circulating monocytes. In lymphoid tissue CD15 reacts with Reed-Sternberg cells of Hodgkins disease and with granulocytes. However, CD15 reacts with only a few tissue macrophages and does not react with dendritic cells.
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Antibody come from
n/a
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Other description
Provided as sterile filtered solution in phosphate buffered saline with 0.08% sodium azide and 0.2% carrier protein. Protein A/G Chromatography
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Clone
ARE
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Antigen antibody binding interaction
Mouse anti CD15 antibody conjugated to FITC Antibody
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Antibody is raised in
Mouse
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Antibody s reacts with
Human
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Antibody s reacts with these species
This antibody doesn't cross react with other species
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Antibody s specificity
No Data Available
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Research interest
CD Marker
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Application
Flow Cytometry
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Antibody s suited for
PBMC: Add10 µl of MAB/10^6 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2¼ to 8¼C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature (20¼C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturerÕs instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
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Storage
4ºC
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Relevant references
1. Skubitz K, Balke J, Ball E, et al. Report on the CD15 cluster workshop. In: Knapp W, Dšrken B, Gilks WR, et al, eds. LeucocyteTyping IV: White Cell Differentiation Antigens. Oxford: Oxford University Press; 1989;800-805. _x000B__x000B_2. Hanjan SNS, Kearney JF, Cooper MD. A monoclonal antibody (MMA) that identifies a differentiation antigen on human myelomonocytic cells. Clin Immunol Immunopath. 1982;23:172. _x000B__x000B_3. Hsu SM, Jaffe ES. Leu-M1 and peanut agglutinin stain the neoplastic cells of Hodgkin's Disease. Amer J Clin Path. 1984;82:29. _x000B__x000B_4. Pinkus GS, Thomas P, Said JW. Leu-M1ÑA marker for Reed Sternberg cells in Hodgkin's Disease: An immunoperoxidase study of paraffin-embedded tissues. Am J Pathol. 1985;119:244.
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus
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French translation
anticorps
