Mouse anti CD10

• Catalog number
  0101
• Price
  Please ask
• Size
  100 μg

• Category
  Primary Antibodies
• Long description
  Anti-hCD10 recognizes a human common acute lymphoblastic antigen (CALLA), MW 100 kDa. The CD10 antigen is identical to human membraneassociated neutral endopeptidase ( NEP;EC 3.3.24.11), also known as enkephalinase. The CD10 antigen is found on lymphocytes from patients with acute B-lymphoid leukemia. The antigen is also present on a wide variety of normal and neoplastic cell types including renal epithellum, fibroblasts, granulocytes and some lymphoma, melanoma and glioma cell lines
• Antibody come from
  n/a
• Other description
  Provided as solution in phosphate buffered saline with 0.08% sodium azide. Protein A/G Chromatography
• Clone
  RXB.14
• Antigen antibody binding interaction
  Mouse anti CD10 Antibody
• Antibody is raised in
  Mouse
• Antibody s reacts with
  see techfile
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Research interest
  CD Marker
• Application
  Flow Cytometry
• Antibody s suited for
  PBMC: Add 10 µl of MAB/10 PBMC in 100 µl PBS. Mix gently and incubate for 15 minutes at 2o to 8o C. Wash twice with PBS and analyze. WHOLE BLOOD: Add10 µl of MAB/100 µl of whole blood. Mix gently and incubate for 15 minutes at room temperature 20o C. Lyse the whole blood. Wash once with PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
• Storage
  -20ºC
• Relevant references
  1. CD10/Neutral Endopeptidase 24.11 Hydrolyzes Bombesin-like Peptides and Regulates the Growth of Small Cell Carcinomas of the Lung. Proc Natl Acad Sci USA 1991, Dec1;88(23):10662-6. Shipp, MA, Tarr, GE; Chen, CY; Switzer, SN; Hersh, LB; Stein,H; Sunday, ME; Reinherz, EL. _x000B__x000B_2 Induction of Isotype Switching and Ig Production by CD5+ and CD10+ Human Fetal B Cells. JI, 1992, Jun1;148(11):3398-404. Punnonen, J; Aversa, GG;Vandekerckhove, B; Roncarolo, MG; de Vries, JE. _x000B__x000B_3. Murine Common Acute Lymphoblastic Leukemia Antigen (CD10 Neutral Endopeptidase 24.11). Molecular Characterization, Chromosomal Localization and Modeling of the Active Site. JI, 1992, May1;148(9):2817-25. Chen, CY; Salles, G; Seldin, MF; Kister, AE; Reinherz, EL; Shipp, MA. _x000B__x000B_4. Childhood Acute Leukemia with t(11;19) (q23;p13). SO. Leukemia, 1991, Dec;5(12):1064-8. Hudson, MM; Raimondi, SC; Behm, FG; Pui, CH. _x000B__x000B_5. CD10 (CALLA)/Neutral Endopeptidase 24.11 Modulates Inflammatory Peptide-Induced Changes in Neutrophil Morphology, Migration and Adhesion Proteins and is itself Regulated by Neutrophil Activation. Blood, 1991, Oct. 1;78(7):1834-41. Shipp, MA; Stefano, GB; Switzer, SN; Griffin, JD; Reinherz, EL. _x000B__x000B_6. Functional Properties of CD19+ B Lymphocytes Positively Selected from Buffy Coats by Immunomagnetic Separation. Funderud, S., Erikstien, B., Asheim, H.C., Nustad ,K., Stokke, T., Blomhoff, H.K., Holte, H., Smeland, E.B.. Eur. J. Immunol. 1990 Ja;20(1):201-6 _x000B__x000B_7. Thymic B Cells from Myasthenia Gravis Patients are Activated B Cells. Phenotypic and Functional Analysis. Leprince, C., Cohen-Kaminsky, S., Berrih-Aknin, S., Vernet-Der Garabedian, B., Treton, D., Galanaud, P., Richard, Y., J. Immunol. 1990 Oct., 145(7):2115-22. _x000B__x000B_8. Prognostic Significance of CD34 Expression in Childhood B Precursor Acute Lymphocytic Leukemia: A Pediatric Onocology Group Study. Borowitz, M.J., Shuster, J.J., Civin, C.I., Carrol, A.J., Look, A.T., Behm, F.G., Land, V.J., Pullen, D.J., Crist, W.M.. J. Clin. Onol. 1990 Au;8(8):1389-98. _x000B__x000B_9. Biphenotypic Acute Leukemia in Adults. Sulak, L.E., Clare, C.N., Morale, B.A., Hansen, K.L., Montiel, M.M.. Am. J. Clin. Path. 1990 Ju;94(1):54-8. _x000B__x000B_10. Intersection of the Complement and Immune Systems: A Signal Transduction Complex of the B Lymphocyte Containing Complement Receptor type 2 and CD19. Matsumoto, A.K., Kopicky-Burd , J., Carter, R.H., Tuveson, D.A., Tedder, T.F., Fearon, D.T., J. Exp. Med. 1991 Jan. 173(1):55-64.
• Protein number
  see ncbi
• Warnings
  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  CD10  
• Gene symbol
  MME
• Short name
  Mouse anti CD10
• Technique
  Mouse, anti, antibody to, mouses
• Host
  mouse
• Isotype
  IgG1
• Label
  unlabeled
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to CD10
• Alternative technique
  murine, antibodies

Gene info

• Identity
  HGNC:7154
• Gene
  MME
• Long gene name
  membrane metalloendopeptidase
• Synonyms
  • CALLA
  • CD10
  • NEP
• Synonyms name
  • neutral endopeptidase
  • enkephalinase
  • neprilysin
• Locus
  3q25.2
• Discovery year
  1989-05-15
• Entrez gene record
  4311
• RefSeq identity
  • NM_000902
• Classification
  • M13 metallopeptidases
  • CD molecules
• VEGA ID
  OTTHUMG00000158455

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

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