Mouse anti Carcinoembryonic Antigen / CEA

  • Catalog number
    MUB0332P
  • Price
    Please ask
  • Size
    0,1 mg
  • Category
    Primary Antibodies
  • Long description
    This monoclonal antibody is reactive with Human carcinoembryonic antigen (CEA), a tumour associated antigen with oncofetal characteristics. Although CEA can be found in tissues of non-neoplastic diseases and normal epithelia, it occurs also in a large variety of carcinomas. Therefore, immunohistochemical detection of CEA is frequently used for the histopathological diagnosis of Human tumours.
  • Antibody come from
    PARLAM 4 is a Mouse monoclonal IgG1 antibody derived by fusion of Sp 2/0 Ag 14 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with isolated Human CEA. The immunogen has been isolated from Human colonic carcinoma cells.
  • Other description
    Each vial contains 100 ul 1 mg/ml purified antibody in PBS containing 0.09% sodium azide.
  • Clone
    PARLAM 4
  • Antigen antibody binding interaction
    Mouse anti Carcinoembryonic Antigen / CEA Antibody
  • Antibody is raised in
    Mouse
  • Antibody s reacts with
    Human
  • Antibody s reacts with these species
    This antibody doesn't cross react with other species
  • Antibody s specificity
    Most polyclonal CEA antisera show cross-reactivity with related antigens such as biliary glycoprotein (BGP) and non-specific cross-reacting antigen 1/11 (NCA). PARLAM 4 does not show cross reactivity, neither with BGP nor with NCA. In immunoblotting the antibody recognizes a single band of 180 kD.
  • Research interest
    Cancer
  • Application
    Flow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
  • Antibody s suited for
    PARLAM 4 is useful for flow cytometry, immunoblotting, immunocytochemistry on methanol fixed cells and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. The antibody is also reactive in formalin-fixed and paraffin-embedded tissue sections after treatment with citRate buffer pH 6.0 in an autoclave. Human colon carcinoma tissue is used as positive control. Optimal antibody dilution should be determined by titration; we recommend a 1:25 – 1:100 dilution for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:500 for immunoblotting appliCation.
  • Storage
    Store at 4°C, or in small aliquots at -20°C.
  • Relevant references
    Verstijnen CP, Arends JW, Moerkerk PT, Warnaar S, Hilgers J, Bosman FT (1986). CEA-specificity of CEA-reactive monoclonal antibodies. Immuno-chemical and immunocytochemical studies. Anticancer Research, 6, 97-104. Henzen-Logmans SC, Schipper NW, Poels LG, Stolk K, Kenemans P, Meyer CJ. (1988) Use of statistical evaluation of antigen profiles in differential diagnosis between colonic and ovarian adenocarcinomas. J.Clin Pathol. 41:644-9. Taal BG, Hageman PC, Delemarre JF, Bonfrèr JM, den Hartog Jager FC. (1992) Metastatic ovarian or colonic cancer: a clinical challenge. Eur J Cancer . 28: 394-9.
  • Protein number
    P06731
  • Warnings
    This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antigens are peptides or recombinant or native dependent on the production method.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus
  • Gene target
  • Gene symbol
    CEACAM7, CEACAM5, PSG2
  • Short name
    Mouse anti Carcinoembryonic Antigen / CEA
  • Technique
    antigen, Mouse, anti, antibody to, antigenes, mouses
  • Host
    mouse
  • Isotype
    IgG1
  • Label
    unlabeled
  • Species
    Mouse, Mouses
  • Alternative name
    Mouse antibody to Carcinoembryonic protein / CEA
  • Alternative technique
    antigenes, murine, antibodies
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    CEA cell adhesion molecule 5
  • Synonyms gene
  • Synonyms gene name
    • carcinoembryonic antigen-related cell adhesion molecule 5
    • carcinoembryonic antigen related cell adhesion molecule 5
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1986-01-01
  • Entrez gene record
  • RefSeq identity
  • Classification
    • I-set domain containing
    • V-set domain containing
    • CEA cell adhesion molecule family
    • CD molecules
  • VEGA ID
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: Experimentation on, or using the organs or tissues from, a human or other mammalian conceptus during the prenatal stage of development that is characterized by rapid morphological changes and the differentiation of basic structures. In humans, this includes the period from the time of fertilization to the end of the eighth week after fertilization.
  • Tree numbers
    • E05.313
  • Qualifiers
    economics, ethics, history, legislation & jurisprudence
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