Mouse anti Carcinoembryonic Antigen / CEA
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Catalog numberMUB0332P
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PricePlease ask
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Size0,1 mg
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CategoryPrimary Antibodies
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Long descriptionThis monoclonal antibody is reactive with Human carcinoembryonic antigen (CEA), a tumour associated antigen with oncofetal characteristics. Although CEA can be found in tissues of non-neoplastic diseases and normal epithelia, it occurs also in a large variety of carcinomas. Therefore, immunohistochemical detection of CEA is frequently used for the histopathological diagnosis of Human tumours.
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Antibody come fromPARLAM 4 is a Mouse monoclonal IgG1 antibody derived by fusion of Sp 2/0 Ag 14 Mouse myeloma cells with spleen cells from a BABL/c Mouse immunized with isolated Human CEA. The immunogen has been isolated from Human colonic carcinoma cells.
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Other descriptionEach vial contains 100 ul 1 mg/ml purified antibody in PBS containing 0.09% sodium azide.
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ClonePARLAM 4
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Antigen antibody binding interactionMouse anti Carcinoembryonic Antigen / CEA Antibody
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Antibody is raised inMouse
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Antibody s reacts withHuman
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Antibody s reacts with these speciesThis antibody doesn't cross react with other species
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Antibody s specificityMost polyclonal CEA antisera show cross-reactivity with related antigens such as biliary glycoprotein (BGP) and non-specific cross-reacting antigen 1/11 (NCA). PARLAM 4 does not show cross reactivity, neither with BGP nor with NCA. In immunoblotting the antibody recognizes a single band of 180 kD.
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Research interestCancer
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ApplicationFlow Cytometry,Immunocytochemistry,Immunohistochemistry (frozen),Immunohistochemistry (paraffin),Western blotting
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Antibody s suited forPARLAM 4 is useful for flow cytometry, immunoblotting, immunocytochemistry on methanol fixed cells and immunohistochemistry on frozen tissues when using a PBS buffer containing 0.1 mM CaCl2 and 0.1 mM MgCl2. The antibody is also reactive in formalin-fixed and paraffin-embedded tissue sections after treatment with citRate buffer pH 6.0 in an autoclave. Human colon carcinoma tissue is used as positive control. Optimal antibody dilution should be determined by titration; we recommend a 1:25 – 1:100 dilution for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 – 1:500 for immunoblotting appliCation.
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StorageStore at 4°C, or in small aliquots at -20°C.
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Relevant referencesVerstijnen CP, Arends JW, Moerkerk PT, Warnaar S, Hilgers J, Bosman FT (1986). CEA-specificity of CEA-reactive monoclonal antibodies. Immuno-chemical and immunocytochemical studies. Anticancer Research, 6, 97-104. Henzen-Logmans SC, Schipper NW, Poels LG, Stolk K, Kenemans P, Meyer CJ. (1988) Use of statistical evaluation of antigen profiles in differential diagnosis between colonic and ovarian adenocarcinomas. J.Clin Pathol. 41:644-9. Taal BG, Hageman PC, Delemarre JF, Bonfrèr JM, den Hartog Jager FC. (1992) Metastatic ovarian or colonic cancer: a clinical challenge. Eur J Cancer . 28: 394-9.
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Protein numberP06731
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This product contains sodium azide. To prevent formation of toxic vapors, do not mix with strong acidic solutions. To prevent formation of potentially explosive metallic azides in metal plumbing, always wash into drain with copious quantities of water. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antigens are peptides or recombinant or native dependent on the production method.
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolCEACAM7, CEACAM5, PSG2
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Short nameMouse anti Carcinoembryonic Antigen / CEA
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Techniqueantigen, Mouse, anti, antibody to, antigenes, mouses
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Hostmouse
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IsotypeIgG1
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Labelunlabeled
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SpeciesMouse, Mouses
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Alternative nameMouse antibody to Carcinoembryonic protein / CEA
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Alternative techniqueantigenes, murine, antibodies
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Gene info
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Identity
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Gene
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Long gene nameCEA cell adhesion molecule 7
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Synonyms gene
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Synonyms gene name
- carcinoembryonic antigen-related cell adhesion molecule 7
- carcinoembryonic antigen related cell adhesion molecule 7
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1991-09-12
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- V-set domain containing
- CEA cell adhesion molecule family
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nameCEA cell adhesion molecule 5
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Synonyms gene
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Synonyms gene name
- carcinoembryonic antigen-related cell adhesion molecule 5
- carcinoembryonic antigen related cell adhesion molecule 5
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Synonyms
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- I-set domain containing
- V-set domain containing
- CEA cell adhesion molecule family
- CD molecules
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namepregnancy specific beta-1-glycoprotein 2
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Synonyms gene
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Synonyms
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Synonyms name
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Locus
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Discovery year1991-06-06
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Pregnancy specific glycoproteins
- V-set domain containing
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VEGA ID
MeSH Data
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Name
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ConceptScope note: Experimentation on, or using the organs or tissues from, a human or other mammalian conceptus during the prenatal stage of development that is characterized by rapid morphological changes and the differentiation of basic structures. In humans, this includes the period from the time of fertilization to the end of the eighth week after fertilization.
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Tree numbers
- E05.313
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Qualifierseconomics, ethics, history, legislation & jurisprudence