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Category
Primary Antibodies
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Long description
The reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal dog plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. As reagent for the direct detection of fibrinogen in dog cells, tissues and body fluids in immunofluorescence; as detection reagent in non-isotopic methodology and solid phase immunochemistry (e.g. ELISA). This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
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Antibody come from
Fibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Other description
Fluorochrome-coupled purified hyperimmune IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2) No preservative added, as it may interfere with the antibody activity.
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Clone
Polyclonal
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Antigen antibody binding interaction
Goat anti Dog fibrinogen antibody conjugated with FITC Antibody
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Antibody is raised in
Goat
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Antibody s reacts with
Canine
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Antibody s reacts with these species
The antiSerum does not cross-react with any other component of Dog plasma. Inter-species crossreactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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Antibody s specificity
Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Goat antiSerum to Dog fibrinogen
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Research interest
Veterinary
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Application
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence
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Antibody s suited for
ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence. The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. It is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.
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Storage
Lyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
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Relevant references
no information yet
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Protein number
see ncbi
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Warnings
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Properties
If you buy Antibodies supplied by nordc they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Canine or Canis Lupus is mostly Beagle used for drug research. nordc produces ELISA test kits and polyclonal antibodies. This nordc Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. nordc FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Latin name
Capra aegagrus hircus
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French translation
anticorps
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Gene target
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Short name
Goat anti fibrinogen antibody conjugated with FITC
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Technique
Antibody, anti, FITC, Goat, antibody to, antibodies against human proteins, antibodies for, antibody Conjugates, Fluorescein, goats
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotype
not specified
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Label
FITC
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Species
Dog, Dogs, Goats
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Alternative name
caprine antibody to canine fibrinogen (antibody to-) coupled including fluorecein
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Alternative technique
antibodies, caprine, fluorescine
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