Goat anti Dog fibrinogen
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Catalog numberGAD/Fbg
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PricePlease ask
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Size1 mL
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CategoryPrimary Antibodies
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Long descriptionThe reactivity of the antiserum is restricted to fibrinogen. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against normal dog plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified fibrinogen. No reaction is obtained with any other plasma protein component or serum. However, the antiserum may also react with fibrin monomers, circulating fibrinopeptides and fibrin degradation products. In precipitating techniques as immunoelectrophoresis and single or double radial immunodiffusion to identify the presence of fibrinogen in dog plasma or other body fluids or to determine its concentration.
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Antibody come fromFibrinogen (clotting factor I) is a heat labile beta glycoprotein present in plasma. It is the precursor of fibrin, which is the key protein constituting the network of the blood clot. Thrombin converts fibrinogen to fibrin by limited proteolysis. Fibrin monomers polymerize to fibrin which is stabilized by cross-linking. Fibrinogen is isolated from fresh plasma after removing prothrombin. Freund’s complete adjuvant is used in the first step of the immunization procedure.
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Other descriptionDelipidated, heat inactivated, lyophilized, stable whole antiserum No preservative added Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
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ClonePolyclonal
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Antigen antibody binding interactionGoat anti Dog fibrinogen Antibody
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Antibody is raised inGoat
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Antibody s reacts withCanine
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Antibody s reacts with these speciesThe antiSerum does not cross react with any other component of Dog plasma. Interspecies cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.
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Antibody s specificityPrecipitating polyclonal Goat antiSerum to Dog fibrinogen
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Research interestVeterinary
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ApplicationImmunoprecipitation
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Antibody s suited forImmunoprecipitation. The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Reconstitute the lyophilized antiserum by adding 1 ml sterile distilled water. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day. Precipitin titre 1:32 when tested against pooled normal dog plasma in agar-block immunodiffusion titration.
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StorageLyophilized at +4° C--at least 10 years. Reconstituted at or below -20° C--3-5 years. Reconstituted at +4° C--7 days
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Relevant referencesno information yet
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Protein numbersee ncbi
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WarningsThis product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving humans or animals. This datasheet is as accurate as reasonably achievable, but Nordic-MUbio accepts no liability for any inaccuracies or omissions in this information.
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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PropertiesCanine or Canis Lupus is mostly Beagle used for drug research. nordc produces ELISA test kits and polyclonal antibodies.
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Latin nameCapra aegagrus hircus
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Gene target
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Gene symbolFGG, FGB, FGA
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Short nameGoat anti fibrinogen
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Techniqueanti, Goat, antibody to, goats
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HostGoat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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Isotypenot specified
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Labelunlabeled
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SpeciesDog, Dogs, Goats
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Alternative namecaprine antibody to canine fibrinogen
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Alternative techniqueantibodies, caprine
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Gene info
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Identity
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Gene
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Long gene namefibrinogen gamma chain
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Synonyms gene name
- fibrinogen, gamma polypeptide
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
- Fibrinogen C domain containing
- Receptor ligands
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namefibrinogen beta chain
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Synonyms gene name
- fibrinogen, B beta polypeptide
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
- Fibrinogen C domain containing
- Receptor ligands
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene namefibrinogen alpha chain
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Synonyms gene name
- fibrinogen, A alpha polypeptide
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Locus
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Discovery year2001-06-22
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Entrez gene record
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RefSeq identity
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Classification
- Fibrinogen C domain containing
- Receptor ligands
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data