Anti-Fade Mounting Media Reagent

• Catalog number
  X2841
• Price
  Please ask
• Size
  7 mL

• Category
  Other Reagents
• Long description
  A permanent mounting media designed for coverslipping specimen slides without requiring alcohol dehydration and xylene clearing steps. Slides are simply mounted from water. Anti-Fade is especially applicable to coverslipping all immunostains (AEC, DAB, Fast Red) as well as all special stains and immunofluorescence stains. It hardens quickly and mounted slide preparations are ready for microscopic examination in less than 10 minutes. Anti-Fade mounting media is formulated for permanent mounting (coverslipping) slides without the need for xylene and alcohol pretreatment steps. Anti-Fade provides permanent preservation of stained tissue sections and other biological slide preparations such as cell smears, cyto-spins for indefinite storage. Anti-Fade is a universal mounting media applicable to permanent preservation of most dyes and chromogens including alcohol soluble chromogens and stains such as AEC, Fast Red, Fast green and BCIP/NBT chromogens. Anti-Fade is also suitable for mounting alcohol insoluble stains such DAB and New Fuchsin. This mounting media is uniquely useful for mounting delicate special stains such as Neutral Fats Stain, crystal violet stain and thioflavin T fluorescent stain. This mounting media is also suitable for mounting immunofluorescence stains. Specimen stained with FITC, R-PE, Cy3, Cy5 Texas Red and other tandem fluorochromes can be coverslipped and preserved with this mounting media.
• Antibody come from
  n/a
• Other description
  Proprietary formulation
• Clone
  not specified
• Antigen antibody binding interaction
  Anti-Fade Mounting Media Reagent
• Antibody is raised in
  see techfile
• Antibody s reacts with
  see techfile
• Antibody s reacts with these species
  This antibody doesn't cross react with other species
• Antibody s specificity
  No Data Available
• Research interest
  Cell Biology
• Application
  Immunostaining
• Antibody s suited for
  The following is a recommended technique for coverslipping slide specimens. Other techniques that achieve the same basic result are equally acceptable. 1. Take the slides from the final wash (for best results water is quite suitable). 2. Remove excess water by air drying, maximum removal of water is recommended. 3. Place the slides down, face up on a flat surface and apply 2-3 drops (or as needed) of Anti-Fade mounting media on the lower edge of the coverslip. 4. Bring the slide up to the edge of the coverslip and invert the slide so that the mounting media touches the slide. Gently complete inversion. 5. For immediate viewing, Quick-Set the coverslip by placing the mounted slides in an oven for the following time: 5 minutes at 37ºC or let the slides sit at room temperature for 10-15 minutes. IMPORTANT: Do not leave slides at 37ºC for more than one hour. Slides left longer than one hour at oven temperature may exhibit small bubble formations. 6. Mounted slides should be properly stored following the microscopic examination. Slides are best stored in the dark and free of dust.
• Storage
  Store at room temperature: Products are stable for one year from purchase when stored properly
• Relevant references
  no information yet
• Protein number
  see ncbi
• Warnings
  For research use only. Not for use in human diagnostics or therapeutics.
• Antibody s link
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. Differentiation and cell culture media are buffered growth medium solutions or culture medium solutions that can be supplied as a solid or liquid designed to support the growth of microorganisms or cells, or small plants. There are different types of media for growing different types of cells.

• Gene target
  Fade   Mounting   Reagent  
• Short name
  Anti-Fade Mounting Reagent
• Technique
  anti-, anti, antibody to, antibodies
• Isotype
  not specified
• Label
  unlabeled
• Alternative name
  Antibody toFade Mounting Media Reagent
• Alternative technique
  antibodies

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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