Zebrafish Gapdh Antibody
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Catalog numberF52573-0.4ML
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Price:
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Size0.4 ml
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CategoryAntibody
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ConcentrationIn 1X PBS, pH 7.4, with 0.09% sodium azide
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenZebrafish Gapdh
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens), Zebrafish ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB, ELISA
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Recommended dilutionsWestern blot: 1:1000
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NotesTitration of the Zebrafish Gapdh antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis Zebrafish Gapdh antibodyis to be used only for research purposes and not for diagnostics..
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UniprotQ5XJ10
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PurityAntigen affinity
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DescriptionHas both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3- phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Modulates the organization and assembly of the cytoskeleton. Also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S- nitrosylation of nuclear target proteins (By similarity).
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ImmunogenThis Gapdh antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 273-298 amino acids from the C-terminal region of zebrafish Gapdh.
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StorageAliquot the Zebrafish Gapdh antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Short nameAnti-Zebrafish Gapdh
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit Ig
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SpeciesZebrafish, Zebrafishes
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Alternative nameAntibodies to Zebrafish Gapdh
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Alternative techniqueantibodies
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MeSH Data
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Name
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ConceptScope note: A technique that localizes specific nucleic acid sequences within intact chromosomes, eukaryotic cells, or bacterial cells through the use of specific nucleic acid-labeled probes.
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Tree numbers
- E01.370.225.500.620.670.325
- E01.370.225.750.600.670.325
- E05.200.500.620.670.325
- E05.200.750.600.670.325
- E05.393.661.475
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Qualifiersethics, mortality, psychology, trends, veterinary, history, classification, economics, instrumentation, methods, nursing, standards, adverse effects, statistics & numerical data