SMAD6 Antibody
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Catalog numberR31249
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Price:
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Size0.1mg
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CategoryAntibody
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Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
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FormAntigen affinity purified
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ConjugationUnconjugated
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ClonePolyclonal antibody
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Recognised antigenSMAD6
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Host animalRabbit (Oryctolagus cuniculus)
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ClonalityPolyclonal (rabbit origin)
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Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applicationsWB
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Recommended dilutionsWestern blot: 0.5-1ug/ml
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NotesThe stated application concentrations are suggested starting amounts. Titration of the SMAD6 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented useThis SMAD6 antibodyis to be used only for research purposes and not for diagnostics..
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UniprotO43541
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PurityAntigen affinity
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DescriptionMothers against decapentaplegic homolog 6 is a protein that in humans is encoded by the SMAD6 gene. It belongs to the SMAD family of proteins, which belongs to the TGFbeta superfamily of modulators. By PCR screening of a somatic cell hybrid panel and YACs, this gene was mapped to 15q22.31. This gene acts as a mediator of TGF-beta and BMP antiflammatory activity. It can suppress 1L 1R-TLR signaling through its direct interaction with PEL1 and prevent NF-kappa-B activation, nuclear transport and NF-kappa-B-mediated expression of proinflammatory genes. SMAD6 may play a role in blocking the BMP-SMAD1 signaling pathway by competing with SMAD4 for receptor-activated SMAD1-binding and binding to regulatory elements in target promoter regions.
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ImmunogenAn amino acid sequence from the C-terminus of human SMAD6 (IKVFDFERSGLQHAPEP) was used as the immunogen for this SMAD6 antibody.
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StorageAfter reconstitution, the SMAD6 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translationanticorps
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Gene target
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Gene symbolSMAD6
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Short nameAnti-SMAD6
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TechniqueAntibody, antibodies against human proteins, antibodies for
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IsotypeRabbit IgG
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Alternative nameAntibodies to SMAD6
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Alternative techniqueantibodies
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Alternative to gene targetSMAD family member 6, AOVD2 and HsT17432 and MADH6 and MADH7, SMAD6 and IDBG-18304 and ENSG00000137834 and 4091, transforming growth factor beta receptor, nuclei, Smad6 and IDBG-175757 and ENSMUSG00000036867 and 17130, SMAD6 and IDBG-645465 and ENSBTAG00000000625 and 511279
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Gene info
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Identity
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Gene
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Long gene nameSMAD family member 6
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Synonyms gene
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Synonyms gene name
- MAD, mothers against decapentaplegic homolog 6 (Drosophila)
- SMAD, mothers against DPP homolog 6 (Drosophila)
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Synonyms
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GenBank acession
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Locus
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Discovery year1997-08-22
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- SMAD family
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data