Shh Antibody / Sonic Hedgehog

• Catalog number
  F53217-0.08ML
• Price
  Please ask
• Size
  0.08 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Shh / Sonic Hedgehog
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, ELISA
• Recommended dilutions
  Western blot: 1:1000-2000
• Notes
  Titration of the Shh antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. The 45 kDa precursor protein autocleaves into 27 kDa amino and 19 kDa carboxy fragments.
• Intented use
  This Shh antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  Q62226
• Purity
  Antigen affinity
• Description
  Sonic hedgehog intercellular signaling is essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Activates the transcription of target genes by interacting with its receptor PTCH1 to prevent normal inhibition by PTCH1 on the constitutive signaling activity of SMO. [UniProt]
• Immunogen
  This mouse Shh antibody was produced from a rabbit immunized with a KLH conjugated synthetic peptide between 58-91 amino acids from the N-terminal region of mouse Sonic hedgehog.
• Storage
  Aliquot the Shh antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Shh   Sonic   Hedgehog  
• Gene symbol
  SHH
• Short name
  Anti-Shh / Sonic Hedgehog
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to Shh / Sonic Hedgehog
• Alternative technique
  antibodies
• Alternative to gene target
  sonic hedgehog, HHG1 and HLP3 and HPE3 and MCOPCB5 and SMMCI and TPT and TPTPS, SHH and IDBG-50464 and ENSG00000164690 and 6469, laminin-1 binding, nuclei, Shh and IDBG-144309 and ENSMUSG00000002633 and 20423, SHH and IDBG-638249 and ENSBTAG00000024552 and

Gene info

• Identity
  HGNC:10848
• Gene
  SHH
• Long gene name
  sonic hedgehog signaling molecule
• Synonyms gene
  • HPE3
  • HLP3
• Synonyms gene name
  • sonic hedgehog (Drosophila) homolog
• Synonyms
  • HHG1
  • SMMCI
  • TPT
  • TPTPS
  • MCOPCB5
• Locus
  7q36.3
• Discovery year
  1995-03-10
• Entrez gene record
  6469
• Pubmed identfication
  • 7590746
• RefSeq identity
  • NM_000193
• Classification
  • Hedgehog signaling molecule family
• VEGA ID
  OTTHUMG00000151349

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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