PPAR alpha Antibody

• Catalog number
  R30482
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  PPAR alpha
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, IHC-F, ICC
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting points. Titration of the PPAR alpha antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This PPAR alpha antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P23204
• Purity
  Antigen affinity
• Description
  Peroxisome proliferator-activated receptor alpha, also known as NR1C1 (nuclear receptor subfamily 1, group C, member 1), is a nuclear receptor protein that in humans is encoded by the PPARA gene. The gene spans 83.7 kb and contains 8 exons and is mapped to chromosome 22q12-q13.1. Sher et al.(1993) cloned a cDNA for human peroxisome proliferator-activated receptor from a human liver cDNA library. The PPAR cDNA exhibited 85% and 91% DNA and deduced amino acid sequence identity, respectively, with mouse PPAR. PPAR alpha is a transcription factor and a major regulator of lipid metabolism in the liver.
• Immunogen
  Amino acids 393-412 (NIGYIEKLQEGIVHVLKLHL-mouse) were used as the immunogen for this PPAR alpha antibody.
• Storage
  After reconstitution, the PPAR alpha antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Additional description
  The Anti-PPAR alpha is a α- or alpha protein sometimes glycoprotein present in blood.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  PPAR   alpha  
• Gene symbol
  PPARA
• Short name
  Anti-PPAR alpha
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to PPAR alpha
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:9232
• Gene
  PPARA
• Long gene name
  peroxisome proliferator activated receptor alpha
• Synonyms gene
  • PPAR
• Synonyms gene name
  • peroxisome proliferative activated receptor, alpha
  • peroxisome proliferator-activated receptor alpha
• Synonyms
  • hPPAR
  • NR1C1
• GenBank acession
  • L02932
• Locus
  22q13.31
• Discovery year
  1993-11-01
• Entrez gene record
  5465
• Pubmed identfication
  • 7684926
  • 10591208
• RefSeq identity
  • NM_001001928
• Classification
  • Peroxisome proliferator activated receptors
• VEGA ID
  OTTHUMG00000150443

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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