Flotillin 1 Antibody

• Catalog number
  R31142
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Flotillin 1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB
• Recommended dilutions
  Western blot: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the Flotillin 1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This Flotillin 1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  O75955
• Purity
  Antigen affinity
• Description
  Flotillin 1 is a protein that in humans is encoded by the FLOT1 gene. The International Radiation Hybrid Mapping Consortium mapped the gene to chromosome 6. Bickel et al.(1997) found that mouse Flot1 behaves as a resident integral membrane protein of caveolae. It consistently copurified with Flot2 and with caveolin-1 in the purification of caveolin-rich membranes. Hazarika et al.(1999) found that stable transfection of Flot1, which they called ESA/flotillin-2, in COS-1 cells induced filopodia formation and changed the epithelial morphology to that of neuronal cells. Santamaria et al.(2005) found that prostate tumor overexpressed gene-1 interacted with Flotillin1 in detergent-insoluble membrane fractions. Flotillin1 colocalized with PTOV1 at the plasma membrane and in the nucleus, and it entered the nucleus concomitant with PTOV1 shortly before initiation of S phase.
• Immunogen
  An amino acid sequence from the middle region of human FLOT1 (KKAAYDIEVNTRRAQA) was used as the immunogen for this Flotillin 1 antibody.
• Storage
  After reconstitution, the Flotillin 1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Flotillin  
• Gene symbol
  FLOT1, FLOT2
• Short name
  Anti-Flotillin 1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Flotillin 1
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:3757
• Gene
  FLOT1
• Long gene name
  flotillin 1
• GenBank acession
  • AF089750
• Locus
  6p21.33
• Discovery year
  2000-05-30
• Entrez gene record
  10211
• Classification
  • Flotillins
• VEGA ID
  OTTHUMG00000031151

Gene info

• Identity
  HGNC:3758
• Gene
  FLOT2
• Long gene name
  flotillin 2
• Synonyms gene
  • M17S1
• Synonyms
  • ESA
  • ESA1
  • ECS-1
  • ECS1
• Synonyms name
  • Flotillin 2 (epidermal surface antigen 1)
  • membrane component, chromosome 17, surface marker 1 (35kD protein identified by monoclonal antibody ECS-1)
• GenBank acession
  • M60922
• Locus
  17q11.2
• Discovery year
  1991-05-09
• Entrez gene record
  2319
• Pubmed identfication
  • 1769667
• RefSeq identity
  • NM_004475
• Classification
  • Flotillins
• VEGA ID
  OTTHUMG00000132674

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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