AQP2 Antibody Aquaporin 2
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Catalog number
R30832
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Price
Please ask
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Size
0.1mg
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Category
Antibody
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Concentration
0.5mg/ml if reconstituted with 0.2ml sterile DI water
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Form
Antigen affinity purified
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Conjugation
Unconjugated
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Clone
Polyclonal antibody
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Recognised antigen
AQP2 Aquaporin 2
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Host animal
Rabbit (Oryctolagus cuniculus)
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Clonality
Polyclonal (rabbit origin)
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Species reactivity
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
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Tested applications
WB, IHC-P, IHC-F
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Recommended dilutions
Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml
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Notes
The stated application concentrations are suggested starting amounts. Titration of the AQP2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
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Intented use
This AQP2 antibodyis to be used only for research purposes and not for diagnostics..
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Uniprot
P41181
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Purity
Antigen affinity
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Description
Aquaporin 2 also called Aquaporin-CD, is found in the apical cell membranes of the kidneys collecting duct principal cells and in intracellular vesicles located throughout the cell. The AQP2 gene is mapped to chromosome 12q13, very close to the site of major intrinsic protein by situ hybridization. Investigators suggest that a defect in the gene is the basis of the autosomal form of nephrogenic diabetes insipidus. The functional expression and the limited localization suggested that AQP2 is the vasopressin-regulated water channel. Using rat kidney slices and porcine kidney cells stably expressing rat Aquaporin 2, trafficking can be stimulated by cAMP-independent pathways that utilize nitric oxide(NO). The NO donors sodium nitroprusside(SNP) and NONOate and the NO synthase substrate L-arginine mimicked the effect of vasopressin(VP), stimulating relocation of Aquaporin 2 from cytoplasmic vesicles to the apical plasma membrane. SNP increased intracellular cGMP rather than cAMP, and exogenous cGMP stimulated AQP2 membrane insertion. Atrial natriuretic factor, which signals via cGMP, also stimulated AQP2 translocation. Expression in kidney connecting tubules is sufficient for survival and that expression in collecting ducts is required to regulate body water balance. The S256L substitution in the cytoplasmic tail of the protein prevented phosphorylation at S256 and the subsequent accumulation of Aquaporin 2 on the apical membrane of the collecting duct principal cells.
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Immunogen
Amino acids 245-260 (DWEEREVRRRQSVELH) were used as the immunogen for this AQP2 antibody (100% homologous in human, mouse and rat).
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Storage
After reconstitution, the AQP2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
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Properties
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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French translation
anticorps
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Gene target
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Gene symbol
AQP2
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Short name
Anti-AQP2 Aquaporin 2
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Technique
Antibody, antibodies against human proteins, antibodies for
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Isotype
Rabbit IgG
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Alternative name
Antibodies to AQP2 Aquaporin 2
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Alternative technique
antibodies
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Alternative to gene target
aquaporin 2 (collecting duct), AQP-CD and WCH-CD, AQP2 and IDBG-32089 and ENSG00000167580 and 359, PDZ domain binding, Plasma membranes, Aqp2 and IDBG-183373 and ENSMUSG00000023013 and 11827, AQP2 and IDBG-632459 and ENSBTAG00000008374 and 539870
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Gene info
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Identity
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Gene
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Long gene name
aquaporin 2
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Synonyms gene name
- aquaporin 2 (collecting duct)
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Locus
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Discovery year
1993-07-09
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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