RAT ANTI HUMAN CD3
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Catalog numberMBS215051
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PricePlease ask
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Size5x0.2 mg
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Products_typeAntibody
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Products_short_name[CD3]
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Other_names[T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e molecule, epsilon (CD3-TCR complex); T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen: CD3e]
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Products_gene_name[CD3]
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Other_gene_names[CD3E; CD3E; T3E; TCRE; IMD18; T3E]
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ClonalityMonoclonal
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Clone[CD3-12]
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ReactivityHuman
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SpecificityRat anti Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3ε chain recognizes human CD3ε. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, γ, δ, ζ) that assemble and function as three pairs of dimers (εγ, εδ, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005).
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FormPurified IgG - liquid
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Storage_stabilityStore at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
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Tested_applicationFlow Cytometry (FC/FACS), Immunohistochemistry - Frozen/Paraffin (IHC-F/P), Immunoprecipitation (IP), Western Blotting (WB), Immunofluorescence (IF)
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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PropertiesHuman proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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Gene target
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Gene symbolCD3G, CD3E
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Short nameANTI CD3
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Techniqueanti human, anti, antibody to, anti human antibodies
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HostRat
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IsotypeIgG1
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SpeciesHuman, Humans, Rats
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Alternative nameRAT antibody to H. sapiens CD3
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Alternative techniqueantibodies
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Gene info
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Identity
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Gene
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Long gene nameCD3g molecule
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Synonyms gene name
- CD3g antigen, gamma polypeptide (TiT3 complex)
- CD3g molecule, gamma (CD3-TCR complex)
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GenBank acession
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Locus
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Discovery year1988-05-11
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Entrez gene record
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RefSeq identity
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Classification
- CD molecules
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VEGA ID
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Locus Specific Databases
Gene info
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Identity
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Gene
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Long gene nameCD3e molecule
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Synonyms gene name
- CD3e antigen, epsilon polypeptide (TiT3 complex)
- CD3e molecule, epsilon (CD3-TCR complex)
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GenBank acession
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Locus
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Discovery year1986-01-01
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Entrez gene record
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RefSeq identity
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Classification
- CD molecules
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VEGA ID
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Locus Specific Databases
MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data