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Products_type
Antibody
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Products_short_name
[VPS34]
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Other_names
[phosphatidylinositol 3-kinase catalytic subunit type 3; Phosphatidylinositol 3-kinase catalytic subunit type 3; phosphatidylinositol 3-kinase catalytic subunit type 3; PI3-kinase type 3; PI3K type 3; ptdIns-3-kinase type 3; phosphoinositide-3-kinase, class 3; Phosphoinositide-3-kinase class 3]
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Other_gene_names
[Pik3c3; Pik3c3; Vps34; 5330434F23Rik; PI3-kinase type 3; PI3K type 3; PtdIns-3-kinase type 3]
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Clonality
Polyclonal
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Reactivity
Mouse, Rat
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Specificity
This item specifically recognises VPS34 (vacuolar protein sorting 34), otherwise known as PI3-kinase type 3 (PIK3C3), a ubiquitously expressed class III PI3K (phosphoinositide 3-kinase), and member of the PI3/PI4-kinase family, which specifically utilizes phosphatidylinositol (PtdIn) as a substrate, and functions as the catalytic subunit of the PI3K complex. VPS34 plays a role in the transport of lysosomal enzyme precursors to lysosomes, and in endocytosis and vesicular trafficking, and has been strongly implicated as a regulator of autophagy, forming a multi-protein complex with the proautophagic tumor suppressors Beclin1/Atg6, Bif-1, and UVRAG, in the initiation of autophagosome formation. Distinct complexes of VPS34 have been shown to regulate endocytic processes vital for late-stage autophagosome-lysosome fusion. This item has been tested in immunofluorescence using HUVEC cells, in conjunction with Hoescht 33342 (blue) nuclear staining.
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Form
Purified Purified IgG - liquid
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Concentration
IgG concentration 1.0mg/ml
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Storage_stability
Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
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Tested_application
Immunofluorescence (IF), Western Blot (WB)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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About
Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
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Latin name
Oryctolagus cuniculus
