Rabbit anti Human Ig kappa light chain (free and bound), conjugated with FITC

  • Catalog number
    MBS571237
  • Price
    Please ask
  • Size
    1 mL
  • Products_type
    Secondary Antibody
  • Products_short_name
    [Rabbit anti Human Ig kappa light chain (free and bound)]
  • Clonality
    Polyclonal
  • Reactivity
    Human
  • Specificity
    Fluorescein isothiocyanate-conjugated IgG fraction of polyclonal Rabbit antiSerum to free and bound Human Ig kappa light chain.
  • Storage_stability
    Store at 4 degree C.
  • Tested_application
    ELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
  • Properties
    This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Conjugation
    Anti-FITC Antibody
  • About
    Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
  • Latin name
    Oryctolagus cuniculus
  • Gene target
    kappa   light   chain   free   bound   conjugated   with  
  • Short name
    Rabbit anti Ig kappa light chain (free bound), conjugated with FITC
  • Technique
    anti human, Rabbit, light, anti, FITC, antibody to, anti human antibodies, antibody Conjugates, Fluorescein
  • Host
    Rabbit, Rabbits
  • Label
    FITC
  • Species
    Human, Humans
  • Alternative name
    production species: rabbit antibody to H. sapiens Ig kappa light epitope (free and bound), coupled including fluorecein
  • Alternative technique
    rabbit-anti, antibodies, fluorescine
MeSH Data
  • Name
  • Concept
    Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
  • Tree numbers
    • E01.370.225.500.607.512.240
    • E01.370.225.750.551.512.240
    • E05.200.500.607.512.240
    • E05.200.750.551.512.240
    • E05.478.583.375
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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