Polio Virus 1,2,3 (FITC)

• Catalog number
  MBS622354
• Price
  Please ask
• Size
  5x1 mL

• Products_type
  Antibody
• Products_short_name
  [Polio Virus 1,2,3]
• Products_name_syn
  [Anti -Polio Virus 1,2,3 (FITC)]
• Clonality
  Polyclonal
• Specificity
  Recognizes poliovirus types 1, 2 and 3.
• Purity
  Purified Purified by ammonium sulfate precipitation.
• Form
  Supplied as a liquid in PBS, 1% BSA, 0.09% sodium azide. Labeled with fluorescein isothiocyanate (FITC).
• Storage_stability
  May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. FITC conjugates are sensitive to light.
• Tested_application
  Immunofluorescence (IF)
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

• Gene target
  Polio   Virus  
• Short name
  Polio Virus 1 2 3 (FITC)
• Technique
  FITC, Fluorescein
• Host
  Goat
• Isotype
  IgG
• Label
  FITC
• Species
  Virus, Viruses
• Alternative name
  Polio Virus 1,2,3 (fluorecein)
• Alternative technique
  fluorescine
• Virus
  polio

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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