Muscarinic Acetylcholine Receptor M2, aa225-359 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007)

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  • Catalog number
    MBS602250
  • Price:

    3914.23 USD

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  • Size
    5x0.1 mg
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  • Products_type
    Antibody
  • Products_short_name
    [Muscarinic Acetylcholine Receptor M2, aa225-359]
  • Products_name_syn
    [Anti -Muscarinic Acetylcholine Receptor M2, aa225-359 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007)]
  • Other_names
    [muscarinic acetylcholine receptor M2; Muscarinic acetylcholine receptor M2; muscarinic acetylcholine receptor M2; 7TM receptor; OTTHUMP00000208393; OTTHUMP00000208394; OTTHUMP00000208395; OTTHUMP00000208396; muscarinic M2 receptor; cholinergic receptor, muscarinic 2, isoform a; cholinergic receptor, muscarinic 2]
  • Other_gene_names
    [CHRM2; CHRM2; HM2; FLJ43243; MGC120006; MGC120007]
  • Clonality
    Monoclonal
  • Clone
    [2Q147]
  • Reactivity
    Human, Monkey, Rat
  • Specificity
    M2 muscarinic acetylcholine receptor. No reactivity with the other subtypes. Species crossreactivity: Human, monkey and rat. Expected to react with most mammalian species.
  • Purity
    Purified Purified immunoglobulin
  • Form
    Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
  • Storage_stability
    May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. IMMUNOHISTOCHEMISTRY PROTOCOL Use on 30 um, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. 1. 3 x 10 minute washes in TBS (with or without 0.25% Triton). 2. Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody). 3. Incubate M9520-13B diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at RT followed by 16 hours at 4 degree C. 4. 3 x 10 minute washes in TBS. 5. Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody). 6. 3 x 10 minute washes in TBS. 7. ABC Elite (1:200) in TBS. 8. 2 x 10 minute washes in TBS. 9. 1 x 10 minute wash in phosphate buffer (no saline). 10. DAB reaction with 0.06% NiCl added for intensification. 11. 2 x 10 minute washes in PBS. 12. 1 x 10 minute wash in phosphate buffer (no saline).
  • Tested_application
    Immunohistochemistry (IHC), Immunocytochemistry (ICC)
  • Description
    The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.
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  • Gene target
    Muscarinic   Acetylcholine   Receptor   aa225-359   Chrm2   CHRM-2   7TM   Receptor   FLJ43243   HM2   MGC120006   MGC120007  
  • Gene symbol
    CHRM2, MIR1289-2, MIR521-2, MIR509-2, MIR512-2, MIR7-2, MIR329-2, RNU6-2, KRTAP13-2, SNORD114-2
  • Short name
    Muscarinic Acetylcholine Receptor M2, aa225-359 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007)
  • Host
    Rat
  • Isotype
    IgG2a
  • Alternative name
    Muscarinic Acetylcholine Receptor M2, aa225-359 (Chrm2, CHRM-2, 7TM Receptor, FLJ43243, HM2, MGC120006, MGC120007)
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Muscarinic Acetylcholine Receptor M2 aa225 359 Chrm2 CHRM 2
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