MOUSE ANTI HUMAN CD100:FITC
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Catalog numberMBS210643
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Price:
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Size0.1 mg
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Products_typeAntibody
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Products_short_name[CD100]
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Other_names[semaphorin-4D isoform 2; Semaphorin-4D; semaphorin-4D; A8; BB18; GR3; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; A8; BB18; GR3; CD_antigen: CD100]
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Products_gene_name[CD100]
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Other_gene_names[SEMA4D; SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G; C9orf164; CD100; SEMAJ]
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ClonalityMonoclonal
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Clone[A8]
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FormFITC Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid from tissue culture supernatant.
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ConcentrationIgG concentration 0.1 mg/ml
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Storage_stabilityStore at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. Shelf Life: 18 months from date of despatch.
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Tested_applicationFlow cytometry (FC/FACS)
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody against the Hu protein or peptide or recombinant supplied in 1 volumes. Ask for quote if you need bulk.
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PropertiesThis MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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ConjugationAnti-FITC Antibody
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TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin nameMus musculus
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Gene target
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Gene symbolSEMA4D
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Short nameMOUSE ANTI CD100:FITC
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Techniqueanti human, Mouse, anti, FITC, antibody to, anti human antibodies, Fluorescein, mouses
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HostMouse
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IsotypeIgG1
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LabelFITC
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SpeciesHuman, Humans, Mouses
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Alternative nameMOUSE antibody to H. sapiens CD100:fluorecein
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Alternative techniquemurine, antibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene namesemaphorin 4D
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Synonyms gene
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Synonyms gene name
- chromosome 9 open reading frame 164
- sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D
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Synonyms
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Synonyms name
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GenBank acession
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Locus
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Discovery year1999-06-25
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Immunoglobulin like domain containing
- Semaphorins
- CD molecules
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VEGA ID
MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data