Mouse anti CD14, conjugated to FITC

• Catalog number
  MBS570371
• Price
  Please ask
• Size
  5x2 mL

• Products_type
  Antibody
• Products_short_name
  [CD14]
• Other_names
  [CD14; Monocyte differentiation antigen CD14; monocyte differentiation antigen CD14; CD14 antigen; myeloid cell-specific leucine-rich glycoprotein; CD14 molecule
• Products_gene_name
  [CD14]
• Other_gene_names
  [CD14; CD14]
• Clone
  [MEM18]
• Reactivity
  Human
• Specificity
  The CD14 mAb (clone MEM18) recognizes surface CD14 on human monocytes and macrophages as well as on neutrophils. The sensitivity of MEM18 mAb is determined by staining well-defined blood samples from representative donors with serial-fold mAb dilutions to obtain a titration curve that allows relating the mAb concentration to the percentage of stained cells and geometric MFI (mean fluorescence intensity). For this purpose, a mAb-concentration range is selected to include both the saturation point (i.e. the mAb dilution expected to bind all epitopes on the target cell) and the detection threshold (i.e. the mAb dilution expected to represent the least amount of mAb needed to detect an identical percentage of cells). In practice, 50 ul of leukocytes containing 10^7 cells/ml are stained with 20 ul mAb of various dilutions to obtain a titration curve and to identify the saturation point and detection threshold. The final concentration of the product is then adjusted to be at least 3-fold above the detection threshold. In addition and to control lot-to-lot variation, the given lot is compared and adjusted to fluorescence standards with defined intensity.
• Form
  PBS pH 7.2, 1% BSA, 0.05% NaN3
• Storage_stability
  Monoclonal antibody reagents contain optimal concentrations of affinity-purified antibody. For stability reasons this monoclonal antibody solution contains sodium azide. These reagents should be stored at 2-8 degree C (DO NOT FREEZE!) and protected from prolonged exposure to light. Stability of the reagent: Please refer to the expiry date printed onto the vial. The use of the reagent after the expiration date is not recommended.
• Tested_application
  Direct Immunofluorescence (IF), Indirect Immunofluorescence (IF), Flow Cytometry (FC/FACS)
• Description
  This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody
• Test
  Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
• Latin name
  Mus musculus

• Gene target
  CD14   conjugated  
• Gene symbol
  CD14
• Short name
  Mouse anti CD14, conjugated FITC
• Technique
  Mouse, anti, FITC, antibody to, antibody Conjugates, Fluorescein, mouses
• Host
  Mouse
• Isotype
  IgG1
• Label
  FITC
• Species
  Mouse, Mouses
• Alternative name
  Mouse antibody to CD14 molecule, coupled to fluorecein
• Alternative technique
  murine, antibodies, fluorescine
• Alternative to gene target
  CD14 molecule, CD14 and IDBG-49157 and ENSG00000170458 and 929, lipoteichoic acid binding, Extracellular, Cd14 and IDBG-138525 and ENSMUSG00000051439 and 12475, CD14 and IDBG-646064 and ENSBTAG00000015032 and 281048

Gene info

• Identity
  HGNC:1628
• Gene
  CD14
• Long gene name
  CD14 molecule
• Synonyms gene name
  • CD14 antigen
• Locus
  5q31.3
• Discovery year
  1988-05-31
• Entrez gene record
  929
• Pubmed identfication
  • 2472171
  • 2462937
• RefSeq identity
  • NM_000591
• Classification
  • CD molecules
  • Scavenger receptors
• VEGA ID
  OTTHUMG00000129507

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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