Macrophages mature, Human, mAb 25F9, FITC

• Catalog number
  MBS584399
• Price
  Please ask
• Size
  0.1 mg

• Products_type
  Antibody
• Products_short_name
  [Macrophages mature]
• Clonality
  Monoclonal
• Reactivity
  Reactivity: Human. Cross reactivity: Rhesus monkey
• Form
  1 ml (100 ug/ml) 0.2um filtered FITC conjugated antibody solution in PBS, containing 1% bovine serum albumin and 0.02% sodium azide.
• Storage_stability
  Product should be stored at 4 degree C. Under recommended storage conditions, product is stable for one year.
• Tested_application
  F, Flow Cytometry (FC/FACS), P, Western Blot (WB
• Properties
  This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Conjugation
  Anti-FITC Antibody

• Gene target
  Macrophages   mature   mAb   25F9  
• Short name
  Macrophages mature, , mAb 25F9, FITC
• Technique
  FITC, Fluorescein
• Host
  Mouse
• Isotype
  Mouse IgG1
• Label
  FITC
• Species
  Human, Humans
• Alternative name
  Macrophages mature, H. sapiens, monoclonal 25F9, fluorecein
• Alternative technique
  fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

Product images

Similar products