IgG, Fc, X-Adsorbed (FITC)
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Catalog numberMBS626495
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Price:
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Size0.5 mg
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Products_typeAntibody
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Products_short_name[IgG, Fc, X-Adsorbed]
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Products_name_syn[Anti -IgG, Fc, X-Adsorbed (FITC)]
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ClonalityPolyclonal
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ReactivityHuman
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SpecificityRecognizes heavy chains on human IgG based on IEP. Minimum crossreactivity with bovine, horse, mouse or rabbit serum proteins.
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PurityAffinity Purified ~90% based on SDS-PAGE., small amounts of intact IgG may be present.
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FormSupplied as a liquid in 10mM sodium phosphate, 0.15M sodium chloride, pH 7.2, 1% BSA, Protease/IgG free, 0.05% sodium azide. Labeled with Fuorescein Isothiocyanate (FITC).
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Storage_stabilityMay be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.
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Tested_applicationImmunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
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PropertiesThis MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Short nameIgG, Fc, X-Adsorbed (FITC)
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TechniqueFITC, IgG, FC, Fluorescein, IgGs
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HostGoat
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IsotypeF(ab')2, IgG
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LabelFITC
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Alternative nameImmunoglobulin G, fragment c, X-Adsorbed (fluorecein)
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Alternative techniquefluorescine, immunoglobulins
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MeSH Data
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Name
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ConceptScope note: A method for the study of certain organic compounds within cells, in situ, by measuring the light intensities of the selectively stained areas of cytoplasm. The compounds studied and their locations in the cells are made to fluoresce and are observed under a microscope.
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Tree numbers
- E01.370.225.500.386
- E05.196.712.516.600.240
- E05.200.500.386
- E05.242.386
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data