Hot Start PCR Ready Mix (2x), monoclonal antibody based hot start[Hot Start PCR]
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Catalog numberMBS430155
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Price:
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SizeNA
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Products_typeAntibody
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PropertiesIf you buy Antibodies supplied by MyBioSource they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR. Hot start taq polymerases can be supplied as mix with dNTPs or Mg+ free Polymerases. The units are redifined for reactions but you can delute more this Hot start TAQ polymerase. Storage at -25 º C.
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AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes. TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
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GroupPCR, polymerase chain reaction
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French translationanticorps
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Gene target
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Short nameHot Start PCR (2x), monoclonal antibody based hot start[Hot Start PCR]
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TechniqueAntibody, PCR, antibodies against human proteins, antibodies for, Monoclonals or monoclonal antibodies, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment.
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Alternative nameHot Start PCR test kit Ready Mix (2x), monoclonal (antibody to-) based hot start[Hot Start PCR test kit]
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Alternative techniqueantibodies, dna-amplification
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MeSH Data
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Name
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ConceptScope note: Laboratory techniques that involve the in-vitro synthesis of many copies of DNA or RNA from one original template.
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Tree numbers
- E05.393.620
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data