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Products_type
Secondary Antibody
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Products_short_name
[IgG (H&L)]
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Products_name_syn
[Goat anti-Rabbit IgG (H&L) - Affinity Pure]
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Reactivity
Rabbit. Cross Reactivity: Based on IEP, no reactivity is observed to: non-immunoglobulin rabbit serum proteins human serum proteins
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Specificity
Based on IEP, this antibody reacts with: heavy (gamma) chains on rabbit IgG, light chains on all rabbit immunoglobulins
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Purity
Affinity purified antibody is > 95% based on SDS-PAGE Affinity purified using solid phase Rabbit IgG.
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Form
Lyophilized
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Concentration
1.0 mg/ml (E 1% at 280 nm = 13.0)
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Storage_stability
Store freeze-dried powder at 2-8 degree C. Shelf Life: Store lyophilized material at 2-8 degree C. For long term storage after reconstitution, dilute with 50% glycerol and store at -20 degree C as a liquid.
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Tested_application
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC)
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Test
A high affinity purification column was use to purify Goat anti-Rabbit IgG (H L) - Pure, Conjugate, min x w/ serum by MyBioSource by chromatographic size exclusion.
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use. The ultrapure Goat anti-Rabbit IgG (H L) - Pure, Conjugate, min x w/ serum supplied from MyBioSource is supplied in 1. The reagent contains very low traces of production synthesis impurities and is highly purified by ultra filtration and distillation for the chemical compounds. Azide Free Serum for cell culture FBS fetal bovine serum. Filtered serum for sterility. The polyclonal serum contains no red or white blood cells or clotting factors; it is the blood plasma not including the fibrinogens. Serum includes all proteins and antibodies not used in blood clotting (coagulation) and all the electrolytes, antibodies, antigens, hormones, and any exogenous substances like drugs and microorganisms. Plasmas on request.
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Latin name
Capra aegagrus hircus, Oryctolagus cuniculus
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Properties
HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot. Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
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Conjugation
HRP Conjugated
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About
Immunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures. Rabbits are used for polyclonal antibody production by MyBioSource. Rabbit antibodies are very stable and can be stored for several days at room temperature. MyBioSource adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long. Serum should be stored at +5°C
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Group
Sera
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Gene target
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Short name
Goat anti-Rabbit IgG (H L) - Pure, Conjugate, min x w/ serum
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Technique
affinity, Rabbit, anti-, serum, anti, Goat, Pure, IgG, antibody to, antibodies, goats, IgGs, pure products
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Host
Goat, The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial. Rabbits
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Isotype
IgG, Heavy & Light chains, IgG
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Label
HRP
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Species
Human, Goats, Humans
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Alternative name
caprine Antibody tohost: rabbit Immunoglobulin G (heavy + light chain) - protein Pure, horseradish peroxidase Conjugate, min x w/ H. sapiens blood serum
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Alternative technique
rabbit-anti, sera, antibodies, purified, caprine, immunoglobulins
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Tissue
serum
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