Donkey anti Goat IgG (heavy and light chains), conjugated with FITC
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Catalog numberMBS571865
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Price:
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Size5x2 mL
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Products_typeSecondary Antibody
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Products_short_name[Donkey anti Goat IgG (heavy and light chains)]
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ClonalityPolyclonal
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ReactivityCaprine
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SpecificityFluorescein isothiocyanate-conjugated IgG fraction of polyclonal Donkey antiSerum to Goat IgG, heavy and light chains
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Storage_stabilityStore at 4 degree C.
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Tested_applicationELISA (EIA), Immunocytochemistry (IHC), Immunohistochemistry (IHC), (In)direct Immunofluorescence (IF)
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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Latin nameEquus africanus asinus, Capra aegagrus hircus
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PropertiesThis MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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AboutImmunoglobulin gamma, IgG, mouse monoclonal H&L chain clones or rabbit, goat polyclonal antibodies have 4 parts. There are 2 heavy chains, 2 light chains. The IgG antibody has 2 antigen binding sites. They represent 70% or more of serum antibodies. This antibody can be antigen purified or protein A or G purified. For storage sodium azide is added or you can call us to request azide free antibody preparations. These will need colder storage temperatures.
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Gene target
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Short nameanti Goat IgG (heavy light chains), conjugated with FITC
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Techniquelight, anti, FITC, Goat, IgG, antibody to, antibody Conjugates, Fluorescein, goats, IgGs
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HostDonkey, Donkeys or Equus africanus asinus are often used as host species to raise antibodies. Even Equus ferus or horse antibodies are different than donkey antibodies since we know horse anti donkey IgGs. For immunohistochemistry it is useful to use an anti-donkey or horse block if your primary antibody is a donkey anti-human. We provide also anti-donkey as a species antibodies and ELISA kits. The goat polyclonal antibodies are directed against human genes or rabbit or mouse IgGs (H +L) chain primary antibodies and conjugated for detection. It are very good secondary antibodies that are affinity purified and have a good affinity themselves too. Often KLH is coupled to the antigen so anti KLH can be present in the vial.
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IsotypeIgG, IgG
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LabelFITC
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SpeciesDonkeys, Goats
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Alternative nameDonkey antibody to caprine Immunoglobulin G (heavy and light chains), coupled including fluorecein
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Alternative techniqueantibodies, caprine, fluorescine, immunoglobulins
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MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data