CD152 IgG2a Fc, Fusion Protein (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4) (FITC)
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Catalog number
MBS634987
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Price
Please ask
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Size
50 Tests
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Other size
please contact us to order other different size
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Description
Antigens are peptides or recombinant or native dependent on the production method. Fusion proteins or chimeric proteins are proteins created through the joining of two or more genes that originally coded for separate proteins. A GFP gene is often used as tag to a reporter gene. Fusion lentiverctors can be used as viral particles to produce proteins that carry for example a GFP tag. Antigen purification of recombinant fusion tag proteins is a frequent strategy using a Fralg tag.
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Properties
This MyBioSource Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MyBioSource FITC is highly sensitive to pH extremes.
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Conjugation
Anti-FITC Antibody
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Gene target
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Gene symbol
CTLA4
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Short name
CD152 IgG2a Fc, Protein (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4) (FITC)
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Technique
antigen, FITC, FC, antigenes, Fluorescein
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Isotype
IgG2a
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Label
FITC
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Alternative name
CD152 IgG2a fragment c, Fusion Protein (cytotoxic T-lymphocyte-associated protein 4, Cytolytic T-lymphocyte-associated protein 4) (fluorecein)
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Alternative technique
antigenes, fluorescine
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Alternative to gene target
cytotoxic T-lymphocyte-associated protein 4, CD and CD152 and CELIAC3 and CTLA-4 and GRD4 and GSE and IDDM12, CTLA4 and IDBG-79215 and ENSG00000163599 and 1493, protein binding, Cell surfaces, Ctla4 and IDBG-159662 and ENSMUSG00000026011 and 12477, CTLA-4 and IDBG-643375 and ENSBTAG00000013170 and 281732
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Tissue
lymphocyte
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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