Escherichia coli, O157:H7 (E. coli) (FITC)

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• Catalog number
  MBS622224
• Price:

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• Size
  500ug

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• Alternative name1
  Anti-Escherichia coli, O157:H7 (E. coli) (FITC)
• Alternative name2
  Escherichia coli, O157:H7 (E. coli) (FITC)
• Alternative name3
  Escherichia coli, O157:H7 (E. coli) (FITC)
• Alternative name4
  Escherichia coli, O157:H7
• Alternative name5
  Anti -Escherichia coli, O157:H7 (E. coli) (FITC)
• Other names
  EscF; N/A
• Gene name
  N/A
• Gene name synonims
  N/A
• Other gene names
  N/A
• Category
  Antibodies
• Clonality
  Polyclonal
• Immunoglobulin isotype
  IgG
• Clone
  Polyclonal antibody
• Host organism
  Goat
• Species reactivity
  E Coli
• Specificity and cross reactivity
  Recognizes E coli 0157:H7.
• Purification method
  Affinity Purified by affinity chromatography.
• Form Appearance
  Supplied as a lyophilized powder. Labeled with FITC. Reconstitute with 1ml sterile ddH2O.
• Concentration
  N/A
• Storage and shipping
  Lyophilized powder may be stored productone should be stored at -20 degrees Celsius.. Stable for 12 months productone should be stored at -20 degrees Celsius.. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, productone should be kept at -20 degrees Celsius. Reconstituted product is stable for 12 months productone should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
• Tested for
  ELISA (EL/EIA), Immunofluorescence (IF)
• Description
  productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
• Advisory
  In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
• Properties
  This MBS Polyclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Polyclonals FITC is highly sensitive to pH extremes.
• Conjugation
  Anti-FITC Antibody

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• Gene target
  Escherichia   coli   O157  
• Short name
  Escherichia coli, O157:H7 ( ) (FITC)
• Technique
  FITC, Fluorescein
• Host
  e. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. MBS Polyclonals supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
• Label
  FITC
• Species
  E. coli, E. coli
• Alternative name
  Escherichia coli, O157:H7 (E. coli) (fluorecein)
• Alternative technique
  escherichia, fluorescine

MeSH Data

• Name
  Fluorescent Antibody Technique
• Concept
  Scope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
• Tree numbers
  • E01.370.225.500.607.512.240
  • E01.370.225.750.551.512.240
  • E05.200.500.607.512.240
  • E05.200.750.551.512.240
  • E05.478.583.375
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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