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Long name
FITC-linked Antibody to Stem Cell Factor (SCF)
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Antigen
Stem Cell Factor (SCF)
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Synonim names
N/A
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Other names
stem cell factor; N/A
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Gene name
Stem Cell Factor (SCF) ; SCF
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Gene synonims
N/A
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Category
Antibodies
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Clonality
Polyclonal Antibodies
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Clone
Not applicable to Polyclonal Antibodies
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Reacts with
Due to limitted amount of tested species we cannot guarantee that no crossreactivity will occur.
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Specificity
The antibody is a rabbit polyclonal antibody raised against SCF conjugated to fitc. It has been selected for its ability to recognize SCF in immunohistochemical staining andwestern blotting. ;This is an antibody designed to detect Stem Cell Factor (SCF) ; SCF
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Purification method
This antibody was purified via Affinity Chromatography
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Form Appearance
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
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Concentration
0.2mg/ml
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Storage shipping and handling
The antibody is shipped at +4 degrees Celsius. Upon receving, freeze at -20. For longer periods of time we recommend keeping the vial frozen at -40 or -80. Avoid cycles of freezing and thawing as they may denaturate the polypeptide chains of the antibody. Due to transportation or handling reasons, small amounts of the antibody might get caught on the lid or walls of the vial. We recommend you to briefly cetrifuge the vial prior to use to gather the content on the bottom.
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Applications
Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blot (WB)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. For cells, cell lines and tissues in culture till half confluency. Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
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Properties
If you buy Antibodies supplied by MBS Polyclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Test
Stem cell factors and stem cell growth factors will produce stem cells or be part of a transdifferentiation process to produce other cells. A cell can transdifferentiate by going back to the naive stem cell stadium or directly into the other cell, helped by the stem cell and transdifferentiationf actors. Stem cell growth factors or stem cell factors are mostly used to produce iPSCs or induced pluripotent stem cells by Jamaka or Thomson factors by using for example 5 Lenti-III-CMV viruses, expressing the Yamanaka iPSC factor set (Oct4, Sox2, Nanog and Lin28) + GFP positive control. Trans differentiation will omit the stem cell stadium but stem cell factors sill play an important role in trans differentiation strategies.
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French translation
anticorps
