Rat Anti-Mouse Ly-6A/E-SPRD

  • Catalog number
    GEN673483
  • Price
    Please ask
  • Size
    100ug
  • Also known as
    Ly-6A/E (Sca-1)
  • Other names
    N/A
  • Category
    Secondary Antibodies
  • Subcategory
    Mnoclonal antibodies
  • Gene name
    N/A
  • Gene name synonims
    N/A
  • Other gene names
    N/A
  • Clonality
    Monoclonal
  • Immunoglobulin isotype
    Rat (Lewis) IgG2a kappa
  • Clone
    D7
  • Host organism
    Rat (Rattus norvegicus)
  • Species reactivity
    Mouse (Mus musculus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
  • Specificity and cross reactivity
    Ly-6A.2 and Ly-6E.1, allelic members of the Ly-6 multigene family; Mr 18 kDa Ly-6A/E, also known as Sca-1, is a member of the Ly-6 multigene family of type V glycophosphatidylinositol-anchored cell surface proteins.^1-3 It is expressed on multipotent hematopoietic stem cells in bone marrow of mice with both the Ly-6.1 and Ly-6.2 allotypes.^4-6 In the periphery, Ly-6A/E exhibits a pattern of expression which is based on differences between the two allotypes. Ly-6.1 strains (e.g., A, BALB/c, CBA, C3H/He, DBA/1, NZB) have few Ly-6A/E^+ resting peripheral lymphocytes, whereas Ly-6.2 strains (e.g., AKR, C57BL, C57BR, C57L, DBA/2, PL, SJL, SWR, 129) have relatively high numbers of Ly-6A/E^+ lymphocytes.^1, 7-9 The expression of Ly-6A/E is dramatically upregulated in all strains upon cellular activation.^1, 8, 9 Studies with MAb D7 suggest that Ly-6A/E may be involved in B and T lymphocyte responses,^9-12 and it appears that the antigen may be required for T-cell receptor-mediated T-cell activation.^3, 13; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
  • Purification method
    N/A
  • Form Appearance
    Spectral Red (SPRD) Conjugate
  • Concentration
    N/A
  • Storage and shipping
    The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The Spectral Red (SPRD) and R-phycoerythrin-Cyanine 5.5 (R-PE-CY 5.5) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store the antibody ats should be kept in the range of 1-7 degrees Celsius.. Do not freeze! The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below Store the antibody at -20 degrees Celsius.. With the exception of the low endotoxin, azide-free product, the vials should be stored at the antibodys should be kept in the range of 1-7 degrees Celsius.. Conjugated forms should not be frozen and should be protected from prolonged exposure to light. Aliquot and freeze the low endotoxin, azide-free product the antibody should be stored at -20 degrees Celsius. immediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored as directed.
  • Tested applications
    Flow Cytometry; Immunohistochemistry (Frozen Sections); Immunoprecipitation; Western Blot
  • Description
    This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
  • Test
    Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
  • Latin name
    Mus musculus, Rattus norvegicus
  • About
    Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
  • Gene target
  • Gene symbol
    CCNL1, PARD6A, HLA-E, SMC2, SNAR-E, IPCEF1, SNRPE, KLRC3, TLE4
  • Short name
    Anti-Mouse Ly-6A/E-SPRD
  • Technique
    anti-, Mouse, anti, antibody to, antibodies, mouses
  • Host
    Rat
  • Label
    SPRD
  • Species
    Mouse, Mouses, Rats
  • Alternative name
    Anti-Rat Anti-Mouse Ly-6A/E-SPRD
  • Alternative technique
    murine, antibodies
Gene info
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    structural maintenance of chromosomes 2
  • Synonyms gene
  • Synonyms gene name
    • SMC2 (structural maintenance of chromosomes 2, yeast)-like 1
    • SMC2 structural maintenance of chromosomes 2-like 1 (yeast)
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    2001-02-22
  • Entrez gene record
  • Pubmed identfication
  • Classification
    • Condensin I subunits
    • Condensin II subunits
    • Structural maintenance of chromosomes proteins
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    small NF90 (ILF3) associated RNA E
  • Synonyms gene name
    • small ILF3/NF90-associated RNA E
  • GenBank acession
  • Locus
  • Discovery year
    2008-07-03
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • Small NF90 (ILF3) associated RNAs
Gene info
Gene info
Gene info
  • Identity
  • Gene
  • Long gene name
    killer cell lectin like receptor C3
  • Synonyms gene name
    • killer cell lectin-like receptor subfamily C, member 3
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1998-01-16
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • C-type lectin domain containing
    • Killer cell lectin like receptors
  • VEGA ID
Gene info
  • Identity
  • Gene
  • Long gene name
    TLE family member 4, transcriptional corepressor
  • Synonyms gene name
    • transducin-like enhancer of split 4 (E(sp1) homolog, Drosophila)
    • transducin like enhancer of split 4
  • Synonyms
  • GenBank acession
  • Locus
  • Discovery year
    1993-12-20
  • Entrez gene record
  • Pubmed identfication
  • RefSeq identity
  • Classification
    • WD repeat domain containing
    • TLE family
    • Wnt enhanceosome complex
  • VEGA ID
MeSH Data
  • Name
  • Concept
    Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
  • Tree numbers
    • E05.196.401.143
    • E05.301.300.096
    • E05.478.566.320.200
    • E05.601.262
    • E05.601.470.320.200
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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