RAT ANTI DYKDDDDK TAG:FITC Antibody
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Catalog numberGEN224499
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Price:
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Size100ug
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Also known asDYKDDDDK TAG
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Other namesN/A
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CategoryAntibodies
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SubcategoryMnoclonal antibodies
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Gene nameN/A
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Gene name synonimsN/A
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Other gene namesN/A
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ClonalityMonoclonal
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Immunoglobulin isotypeIgG1
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Clone6F7
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Host organismRat (Rattus norvegicus)
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Species reactivitySynthetic Peptide; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivityDYKDDDDK TAG This item is specific for the hydrophilic epitope tagging octapeptide sequence DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys), when fused to either the amino- or carboxyl- terminus or at an internal site on target proteins. _x000D__x000D_Clone 6F7 works at neutral pH and has very low background staining in immunocytochemistry.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification methodN/A
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Form AppearanceFITC (Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid)
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ConcentrationIgG concentration 0.1 miligram per 1 mililiter
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Storage and shippingStore the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. the antibody is photosensitive and should be protected from light. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
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Tested applicationsFlow Cytometry (FACS), Immunofluorescence (IF)
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DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
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PropertiesIf you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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Latin nameRattus norvegicus
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French translationanticorps
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Gene target
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Gene symbolLINC01194, CNTN2, TRL-TAG3-1, TRL-TAG1-1, TRL-TAG4-1, TRL-TAG2-1
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Short nameANTI DYKDDDDK TAG:FITC Antibody
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TechniqueAntibody, anti, FITC, antibody to, antibodies against human proteins, antibodies for, Fluorescein, Tags
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HostRat
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Labeltag
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SpeciesRat, Rats
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Alternative nameAnti-RAT ANTI DYKDDDDK TAG:FITC
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Alternative techniqueantibodies, fluorescine
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Gene info
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Identity
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Gene
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Long gene namelong intergenic non-protein coding RNA 1194
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Synonyms gene
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Synonyms gene name
- cancer/testis antigen 49
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Synonyms
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Synonyms name
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Locus
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Discovery year2012-11-30
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Long intergenic non-protein coding RNAs
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VEGA ID
Gene info
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Identity
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Gene
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Long gene namecontactin 2
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Synonyms gene
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Synonyms gene name
- contactin 2 (axonal)
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Synonyms
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GenBank acession
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Locus
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Discovery year2000-05-26
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Entrez gene record
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Pubmed identfication
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RefSeq identity
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Classification
- Contactins
- I-set domain containing
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VEGA ID
Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon TAG) 3-1
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 7 (anticodon UAG)
- transfer RNA-Leu (TAG) 3-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon TAG) 1-1
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Synonyms gene
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Synonyms gene name
- tRNA leucine 2
- tRNA leucine 2 (anticodon UAG)
- transfer RNA leucine 2 (anticodon UAG)
- transfer RNA-Leu (TAG) 1-1
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Synonyms
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GenBank acession
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Locus
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Discovery year1989-06-30
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Entrez gene record
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Pubmed identfication
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Classification
- Cytoplasmic transfer RNAs
Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon TAG) 4-1
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 40 (anticodon UAG) pseudogene
- transfer RNA-Leu (TAG) 4-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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RefSeq identity
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Classification
- Cytoplasmic transfer RNA pseudogenes
Gene info
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Identity
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Gene
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Long gene nametRNA-Leu (anticodon TAG) 2-1
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Synonyms gene
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Synonyms gene name
- transfer RNA leucine 15 (anticodon UAG)
- transfer RNA-Leu (TAG) 2-1
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Synonyms
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GenBank acession
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Locus
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Discovery year2008-08-29
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Entrez gene record
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Classification
- Cytoplasmic transfer RNAs
MeSH Data
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Name
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ConceptScope note: Test for tissue antigen using either a direct method, by conjugation of antibody with fluorescent dye (FLUORESCENT ANTIBODY TECHNIQUE, DIRECT) or an indirect method, by formation of antigen-antibody complex which is then labeled with fluorescein-conjugated anti-immunoglobulin antibody (FLUORESCENT ANTIBODY TECHNIQUE, INDIRECT). The tissue is then examined by fluorescence microscopy.
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Tree numbers
- E01.370.225.500.607.512.240
- E01.370.225.750.551.512.240
- E05.200.500.607.512.240
- E05.200.750.551.512.240
- E05.478.583.375
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data