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Also known as
NEUROFILAMENT M
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Other names
neurofilament medium polypeptide isoform 1; Neurofilament medium polypeptide; neurofilament medium polypeptide; neurofilament 3; 160 kDa neurofilament protein; neurofilament triplet M protein; neurofilament-3 (150 kD medium); neurofilament, medium polypeptide 150kDa; neurofilament, medium polypeptide; 160 kDa neurofilament protein; Neurofilament 3; Neurofilament triplet M protein
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
NEFM; NEFM; NFM; NEF3; NF-M; NEF3; NFM; NF-M
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Gene name synonims
NEFM; NEFM; NFM; NEF3; NF-M; NEF3; NFM; NF-M
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Other gene names
NEFM; NEFM; NFM; NEF3; NF-M; NEF3; NFM; NF-M
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1
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Clone
3H11
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Host organism
Mouse (Mus musculus)
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Species reactivity
Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
NEUROFILAMENT M This item recognises the carboxy terminus of neurofilament M (NF-M), one of three neurofilament proteins (NF-H, NF-M and NF-L). Neurofilaments are 10nm or intermediate filaments found specifically in neurons, with especially high concentrations along the axons, where they appear to control axonal diameter. It is thought that phosphorylation of NF-M results in the formation of interfilament cross-bridges that are important in the maintenance of axonal calibre. Clone 3H11 has also been determined to bind to a C- terminal epitope of bovine NF-M; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
N/A
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Form Appearance
S/N (Tissue Culture Supernatant - liquid)
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short-term storage and at -20 degrees Celsius for long-term.Storage in frost-free freezers is not recommended. the antibody should be stored undiluted. Repeated freeze - thaw cycles may denature the peptide chains of the antibody and therefore should be maximally avoided. If there is a precipitate in the vial we recommend you to briefly microcentrifugate it prior to use. Shelf Life: 18 months from date of dispatch.
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Tested applications
Western Blot (WB), Immunofluorescence (IF)
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Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided. Antibody for research use.
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Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
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Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
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Latin name
Mus musculus, Rattus norvegicus
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About
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
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French translation
anticorps
