Escherichia coli, O157 (E. coli) (FITC) Antibody
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Catalog numberGEN632583
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Price:
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Size100ug
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Also known asEscherichia coli, O157
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Other namesN/A
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CategoryAntibodies
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SubcategoryMnoclonal antibodies
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Gene nameN/A
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Gene name synonimsN/A
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Other gene namesN/A
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ClonalityMonoclonal
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Immunoglobulin isotypeIgG2b
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Clone5E+278
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Host organismMouse (Mus musculus)
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Species reactivityN/A; Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivitySpecific for E. coli serotype O157. Non-reactive with E. coli serotypes: O111, O125, O20, O55 & K12.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification methodAffinity > 90% pure mouse monoclonal antibody which has been purified from ascites fluid or culture
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Form AppearanceSupplied as a liquid in PBS, pH 7.2, 0.1% sodium azide. No stabilizing proteins have been added. Conjugated to FITC.
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ConcentrationN/A
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Storage and shippingStore the antibody at +4 degrees Celsius for short term storage.. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for at least 6 months the antibody should be stored at -20 degrees Celsius.. Prior to use, briefly centrifuge the original vial after thawing and before lid. If further dilution(s) are needed, they can be done with the appropriate assay buffer.
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Tested applicationsELISA (EL/EIA), Immunofluorescence (IF)
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PropertiesIf you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. This MBS Monoclonals Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. MBS Monoclonals FITC is highly sensitive to pH extremes.
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ConjugationAnti-FITC Antibody
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French translationanticorps
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Gene target
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Short nameEscherichia coli, O157 ( ) (FITC) Antibody
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TechniqueAntibody, FITC, antibodies against human proteins, antibodies for, Fluorescein
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Hoste. coli, Escherichia coli, Recombination of bioactive proteins and longer peptides in Escherichia Coli is done often with His tagging. MBS Monoclonals supplies Rec. E. Coli affinity purified or tag purified antigens in 1 quantities or bulk volumes on request.
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LabelFITC
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SpeciesE. coli, E. coli
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Alternative nameAnti-Escherichia coli, O157 (E. coli) (FITC)
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Alternative techniqueantibodies, escherichia, fluorescine
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MeSH Data
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Name
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ConceptScope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiersethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data