CD152, Blocking (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4) (Biotin)
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Catalog number
GEN604019
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Price
Please ask
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Size
100ug
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Also known as
CD152, Blocking
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Other names
N/A
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Category
Antibodies
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Subcategory
Mnoclonal antibodies
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
N/A
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Clonality
Monoclonal
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Immunoglobulin isotype
IgG1k
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Clone
5D3
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Host organism
Mouse (Mus musculus)
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Source organism
Human (Homo sapiens)
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Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
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Specificity and cross reactivity
Recognizes an extracellular epitope of human CTLA-4 expressed on activated T cells. Blocks binding of CD152 (CTLA-4) Ig fusion protein to its CD80/CD86 receptor. Detects immobilized recombinant CD152 IgG2a Fc fusion protein in ELISA.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
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Purification method
Affinity Purified by Protein A affininty chromatography.
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Form Appearance
Supplied as a liquid in PBS, pH 7.5, 5% glycerol, 0.2% BSA, 0.04% sodium azide. Labeled with Biotin.
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Concentration
N/A
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Storage and shipping
Store the antibody at +4 degrees Celsius for short term storage.. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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Tested applications
ELISA (EL/EIA), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
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Description
Antigens are peptides or recombinant or native dependent on the production method.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
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Test
You can block the antibody by the specific target amino acid sequence of peptide.
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Gene target
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Gene symbol
CTLA4
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Short name
CD152, Blocking (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4) ( )
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Technique
Blocking, antigen, antigenes, blocking peptides
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Label
Biotin
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Alternative name
Anti-CD152, Blocking (CTLA4, Cytolytic T-lymphocyte-associated Antigen 4) (Biotin)
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Alternative technique
control, antigenes
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Alternative to gene target
cytotoxic T-lymphocyte-associated protein 4, CD and CD152 and CELIAC3 and CTLA-4 and GRD4 and GSE and IDDM12, CTLA4 and IDBG-79215 and ENSG00000163599 and 1493, protein binding, Cell surfaces, Ctla4 and IDBG-159662 and ENSMUSG00000026011 and 12477, CTLA-4 and IDBG-643375 and ENSBTAG00000013170 and 281732
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Tissue
lymphocyte
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Gene info
MeSH Data
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Name
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Concept
Scope note:
Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
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Tree numbers
- E05.196.401.143
- E05.301.300.096
- E05.478.566.320.200
- E05.601.262
- E05.601.470.320.200
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Qualifiers
ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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