lenti dCAS-VP64_Blast vector

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Description

Lenti kit or vector with CMV or EF promotors for producing Lentivirus (lenti-, Latin for "slow") or lentiviral particles in 293t cells. Lentiviruses are characterized by a long incubation period and often Puro selected. Lentiviruses can deliver a significant amount of viral RNA into the DNA of the host cell and have the unique ability among retroviruses of being able to infect non-dividing cells. They are one of the most efficient methods of a gene delivery vector. The are packaged in HEK 293T cells to get the highest possible lentiviral titers of 108 IFU/ml. The Luc reporter is the expression of the firefly luciferase gene. Puro stands for puromicin selection.

Gene target

dCAS-VP64 Blast vector
Short name

dCAS-VP64_Blast vector
Technique

lenti, Lentivectors and viral particles, Vectors
Alternative name

lentiviral dCAS-VP64_Blast integrating Desoxyribonucleic acid sequence
Virus

lenti

Quantity:

Name

Transduction, Genetic
Concept

Scope note: The transfer of bacterial DNA by phages from an infected bacterium to another bacterium. This also refers to the transfer of genes into eukaryotic cells by viruses. This naturally occurring process is routinely employed as a GENE TRANSFER TECHNIQUE.
Tree numbers
- E05.393.350.800
Qualifiers

ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data