Human Epstein Barr Virus Induced Protein 3 (EBI3) CLIA Kit, 96 well plate

  • Catalog number
    EKU10047
  • Price
    Please ask
  • Size
    96T
  • Verified reactivity
    Homo sapiens (Human)
  • Detection limits
    31.25-2000 pg/mL
  • Sensitivity limit
    1,12 pg/mL
  • Samples to be analyzed
    Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
  • Assay duration
    2 hours, 40min
  • Verified applications
    CLIA
  • Assay class
    Sandwich
  • ELISA detection
    Chemiluminescent
  • Protein number
    Q14213
  • Alternate protein number
    Please refer to SwissProt
  • Gene number
    10148
  • Gene name
    Epstein Barr Virus Induced Protein 3
  • Alternate gene name
    IL27B; IL27-B; Interleukin 27B; Epstein-Barr virus-induced gene 3 protein; EBV-induced gene 3 protein
  • Estimated production time
    7-11 business days
  • Shipping requirements
    Blue ice
  • Storage recommendation
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
  • Use for
    8 months
  • ELISA s specificity
    This assay has high sensitivity and excellent specificity for detection of Epstein Barr Virus Induced Protein 3 (EBI3). No significant cross-reactivity or interference between Epstein Barr Virus Induced Protein 3 (EBI3) and analogues was observed.
  • ELISA s cross reactivity
    This assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
  • Assay principle
    The microplate provided in this kit has been pre-coated with an antibody specific to Epstein Barr Virus Induced Protein 3 (EBI3). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Epstein Barr Virus Induced Protein 3 (EBI3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Epstein Barr Virus Induced Protein 3 (EBI3) level in the sample or standard.;
  • Protocol
    Please see ELISA's datasheet, otherwise contact us
  • Precision of the test
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Epstein Barr Virus Induced Protein 3 (EBI3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Epstein Barr Virus Induced Protein 3 (EBI3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
  • ELISA s stability
    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
  • QC
    The Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
  • Research main area
    Infection immunity;
  • Warnings
    Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
  • Notes
    For research use only. Not for diagnostic procedures.
  • Properties
    Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
  • Description
    Induced protein genes, factors or kinases, increase the production of (an enzyme or other protein) at the level of genetic transcription. A microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
  • Gene target
    Epstein   Barr   Virus   Protein   EBI3   CLIA   Kit  
  • Gene symbol
    EBI3
  • Short name
    Epstein Barr Virus Protein 3 (EBI3) CLIA Kit, 96
  • Species
    Virus, Humans, Viruses
  • Alternative name
    H. sapiens Epstein Barr Virus Induced Protein 3 (Epstein-Barr virus induced 3) CLIA reagent, 96 well plate
  • Alternative technique
    kits
  • Alternative to gene target
    Epstein-Barr virus induced 3, IL-27B and IL27B, EBI3 and IDBG-18580 and ENSG00000105246 and 10148, interleukin-27 receptor binding, Extracellular, Ebi3 and IDBG-191286 and ENSMUSG00000003206 and 50498, EBI3 and IDBG-644295 and ENSBTAG00000012829 and 514933
  • Virus
    epstein
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