TF, Transferrin, Biotin conjugated

  • Catalog number
    IMS06-020-323
  • Price
    Please ask
  • Size
    100 µl (1 µg/µl)
  • Available ordering format
    Liquid in 0.15M sodium chloride, 0.02M sodium phosphate, 0.1% sodium azide, pH 7.2
  • Immunogen
    purified full length native protein P02787
  • Raised in
    Hen
  • Clonality
    Polyclonal
  • Clone
    Polyclonal
  • Purification
    Affinity purified IgY
  • How to reconstitute
    See included datasheet or contact our support service
  • Storage condition
    store at 4°C; make aliquots to avoid working with a stock. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from liquid material adhering to the cap or sides of the tubes.
  • Verified applications
    Western blot (WB)
  • Connected products
    IMS02-020-322 | TF | Transferrin, hen antibodyAS10 1370 | TF | Transferrin, goat antibody
  • Recommended dilutions for use
    1: 5000 (WB)
  • Molecular weight expected аpparent
    77 kDa
  • Verified reactivity
    human
  • Possible reactivity
    primates
  • No reactivity
    no confirmed exceptions from predicted reactivity known in the moment
  • Supplementary information
    prior to conjugation: Only one precipitin arc in immunodiffusion against normal human serum, dilution 1:10, or human transferrin. Only one precipitin arc in immunoelectrophoresis against normal human serum, or human transferrin. Specific against human transferrin in electroblotting when tested against normal human serum.
  • References
    to be added when available
  • Scientific context
    Transferrin is a single polypeptide chain glycoprotein and is a member of the iron binding family of proteins. It has a molecular weight of 77 kDa and a serum concentration range of 1800 to 2700 mg/L. It is synthesised in the liver and consists of two domains, each having a high affinity reversible binding site for Fe3+. The iron is transported in blood and interstitial fluids to sites of use and disposal. Iron/transferrin is essential in haemoglobin synthesis and for certain types of cell division. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. The function of transferrin is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. In addition to its function in iron transport, this protein may also have a physiologic role as granulocyte/pollen binding protein (GPBP) involved in the removal of certain organic matter/allergins from serum.
  • Notes
    None
  • Protein number
    P02787
  • TAIR number
    Refer to NCBI
  • Properties
    Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
  • Conjugation
    Biotinylated
  • Gene target
  • Short name
    TF, Transferrin, conjugated
  • Technique
    antibody Conjugates
  • Label
    Biotin
  • Alternative name
    TF, Transferrin, biotinilated coupled
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