CD68 Exo-Flow capture kit (Magnetic streptavidin beads, CD68-biotin capture antibody, Wash and Elution Buffers, Exo-FITC stain)

• Catalog number
  EXOFLOW620A-1
• Price
  Please ask
• Size
  10 reactions

• Shipping Temperature
  Blue Ice
• Product category
  Exosomes
• Properties
  If you buy Antibodies supplied by Systembio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C. Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6. This Systembio Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Systembio FITC is highly sensitive to pH extremes.
• Conjugation
  Biotinylated, Anti-FITC Antibody, Magnetic beads
• Test
  Flow cytometry  uses monoclonal antibodies of specific affinity clones for cell counting, cell sorting and biomarker detection by suspending cells in a stream of fluid for Forward Scatter, FSC and side scatter, SSC analysis. Human PBMCs can be loaded with CFSE tracking dye after non adherent cell harvesting. Subsequently labeled with anti-CD antibodies, and analyzed by multiparameter flow cytometry. Two-parameter profiles of CD vs. CFSE; and another CD vs. FSC-W. We suggest to use FSC-H vs. FSC-A. FSC-A, FSC-H, FSC-W = area, height, and width of the forward 488 nm light scatter from the flow signal.
• French translation
  anticorps

• Gene target
  CD68   Exo   capture   kit   Magnetic   streptavidin   CD68   capture   Elution   Buffers   Exo   stain  
• Gene symbol
  CD68
• Short name
  CD68 Exo- capture kit (Magnetic streptavidin , CD68- capture antibody, Elution Buffers, Exo-FITC stain)
• Technique
  Antibody, FITC, Flow, antibodies against human proteins, antibodies for, All kind of bead conjugates are available, magnetic, borosilicate, agarose protein A. magnetic beads can be isolated by a macs type magnet. Fluorescein
• Label
  Magnetic
• Alternative name
  CD68 molecule Exo-Flow capture reagent (Magnetic biotinilated-binding streptavidin particles, CD68 molecule-biotinilated capture (antibody to-), washing and Elution Buffers, Exo-fluorecein staining)
• Alternative technique
  antibodies, fluorescine, kits
• Alternative to gene target
  v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, C-Kit and CD117 and PBT and SCFR, KIT and IDBG-18980 and ENSG00000157404 and 3815, transferase activity, Extracellular, Kit and IDBG-172083 and ENSMUSG00000005672 and 16590, KIT and IDBG-642326 and ENSBTAG00000002699 and 280832

Gene info

• Identity
  HGNC:1693
• Gene
  CD68
• Long gene name
  CD68 molecule
• Synonyms gene name
  • CD68 antigen
• Synonyms
  • SCARD1
  • macrosialin
  • GP110
  • DKFZp686M18236
  • LAMP4
• Synonyms name
  • scavenger receptor class D, member 1
  • CD68 antigen
  • macrophage antigen CD68
• GenBank acession
  • S57235
• Locus
  17p13.1
• Discovery year
  1993-06-11
• Entrez gene record
  968
• Pubmed identfication
  • 9790779
• RefSeq identity
  • NM_001251
• Classification
  • Scavenger receptors
  • Lysosome associated membrane proteins
  • CD molecules
• VEGA ID
  OTTHUMG00000108146

MeSH Data

• Name
  Flow Cytometry
• Concept
  Scope note: Technique using an instrument system for making, processing, and displaying one or more measurements on individual cells obtained from a cell suspension. Cells are usually stained with one or more fluorescent dyes specific to cell components of interest, e.g., DNA, and fluorescence of each cell is measured as it rapidly transverses the excitation beam (laser or mercury arc lamp). Fluorescence provides a quantitative measure of various biochemical and biophysical properties of the cell, as well as a basis for cell sorting. Other measurable optical parameters include light absorption and light scattering, the latter being applicable to the measurement of cell size, shape, density, granularity, and stain uptake.
• Tree numbers
  • E01.370.225.500.363.342
  • E01.370.225.500.386.350
  • E05.196.712.516.600.240.350
  • E05.200.500.363.342
  • E05.200.500.386.350
  • E05.242.363.342
  • E05.242.386.350
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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