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Alternative name1
Anti-Semaphorin 3F (Biotin)
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Alternative name2
Semaphorin 3F (Biotin)
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Alternative name3
Semaphorin 3F (Biotin)
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Alternative name4
Semaphorin 3F
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Alternative name5
Anti -Semaphorin 3F (Biotin)
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Other names
semaphorin-3F; Semaphorin-3F; semaphorin-3F; sema IV; sema III/F; semaphorin IV; semaphorin III/F; OTTHUMP00000210657; OTTHUMP00000210658; OTTHUMP00000210669; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, 3F; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F; Sema III/F; Semaphorin IV
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Gene name
N/A
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Gene name synonims
N/A
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Other gene names
SEMA3F; SEMA3F; SEMA4; SEMAK; SEMA-IV; N/A
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Category
Antibodies
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Clonality
Polyclonal
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Immunoglobulin isotype
IgG
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Clone
Polyclonal antibody
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Host organism
Host: Sheep; Source: Human
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Species reactivity
Human, Mouse
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Specificity and cross reactivity
Recognizes human Semaphorin 3F. Species Crossreactivity: mouse.
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Purification method
Affinity Purified by immunoaffinity chromatography.
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Form Appearance
Supplied as a lyophilized powder from PBS, 50ug/1ug BSA. Labeled with Biotin. Reconstitute with sterile TBS, pH 7.3, 20mM Trizma, 150mM sodium chloride, 0.1% BSA.
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Concentration
N/A
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Storage and shipping
Lyophilized powder may be stored productone should be stored at -20 degrees Celsius.. Stable for 12 months productone should be stored at -20 degrees Celsius.. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, productone should be kept at -20 degrees Celsius. Reconstituted product is stable for 12 months productone should be stored at -20 degrees Celsius.. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
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Tested for
Western Blot (WB)
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Description
productone is a polyclonal antibody of high purity and binding affinity for the antigen that it is risen against. Properly used, this antibody will ensure excellent and reproducible results with guaranteed success for the applications that it is tested in. Polyclonal antibodies have series of advantages - larger batches can be supplied at a time, they are inexpensive to manufacture and respectively to buy, the time needed for production is considerably shorter. Polyclonal antibodies generally are more stable and retain their reactivity under unfavorable conditions. To obtain more detailed information on productone, please, refer to the full product datasheet.
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Advisory
In order to retain the quality and the affinity of productone unchanged, please, avoid cycles of freezing and thawing. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
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Properties
Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.
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Conjugation
Biotinylated
